Amano Enzyme Inc.

Akiko Matsunaga

Shotaro Yamaguchi

Hitoshi Amano

Masamichi Okada

Hirotaka Matsubara

Bunzo Mikami

Ryota Hashizume

To provide novel &#x3b2;-amylase derived from a microorganism and a gene thereof. &#x3b2;-amylase derived fromis provided.

Beta-amylase, gene coding therefor and manufacturing method thereof

A novel &#x3b2;-amylase having excellent practical applicability is found, and an object is to provide a practical use of the &#x3b2;-amylase. Provided is a method for improving a food, wherein a &#x3b2;-amylase obtained fromis acted on a food containing a polysaccharide or an oligosaccharide having an &#x3b1;-1,4 bond of glucose as the main chain.

Method for improvement of foods utilizing &#x3b2;-amylase

An object is to provide a novel method for improving an enzyme capable of deamidating a protein. A mutant protein deamidase is designed by the following steps: (A) identifying one or more amino acid in an amino acid sequence for a protein deamidase which corresponds to the amino acid at position 35, 38 to 43, 45, 46, 49, 79 to 84, 103 to 106, 117, 142, 143, 146, 166, or 185 in the amino acid sequence set forth in SEQ ID NO: 2; and (B) constructing a mutant amino acid sequence of the protein deamidase by substituting the one or more amino acid identified in step (A) with another amino acid or other amino acids or by deleting the one or more amino acid identified in step (A).

Method for designing mutant protein deamidase

&#x3b2;-amylase, gene coding therefor and manufacturing method thereof

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Kakamigahara, Japan

Akiko Matsunaga