Takara Bio Inc.

Akiko Kato

Ikunoshin Kato

Hiroaki Sagawa

Tatsuji Enoki

Nobuko Muraki

Takahiro Marui

Kinuko Nagamine

Eiji Kobayashi

Mitsuko Ideno

Fuyuko Takashima

A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.

Method of producing lymphocytes

A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.

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Otsu, Japan

Akiko Kato