Kyushu University

Akihito Harada

Hiroshi Kimura

Yuko Sato

Hitoshi Kurumizaka

Yoko Hayashi

Yasuyuki Ohkawa

Tetsuya Handa

A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.

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Fukuoka, Japan

Akihito Harada