Kaneka Corporation

Akihisa Kanda

Yasuyoshi Ueda

Shiro Kitamura

Kazuyoshi Yajima

Takahisa Kato

Kento Kanaya

Yasuyuki Suzuki

Masao Sato

Wan Ying Lee

Kazuya Yokoe

A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more.

Method for producing coenzyme Q10

A process for producing on an industrial scale the oxidized coenzyme Q, includes culturing a reduced coenzyme Q-producing microorganism selected from the group consisting of the genus, the genus, the genusand the genus, to obtain microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q; and one of: (a) oxidizing thus-obtained reduced coenzyme Qto oxidized coenzyme Qand then extracting the oxidized coenzyme Qby an organic solvent; or (b) extracting reduced coenzyme Qby an organic solvent and oxidizing the extracted reduced coenzyme Qto oxidized coenzyme Q.

Process for producing coenzyme Q10

The present invention relates to a process for producing reduced coenzyme Qwhich comprises obtaining microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q, optionally disrupting the cells and recovering thus produced reduced coenzyme Q. The present invention also relates to a process for producing oxidized coenzyme Qwhich comprises either recovering oxidized coenzyme Qafter oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Qfrom the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Qthereafter. According to the processes of the present invention, reduced coenzyme Qand oxidized coenzyme Qcan be produced simply on the industrial scale.

[object Object]

Provided is a production method of a lipophilic bioactive substance, which includes mixing an aqueous suspension of a microbial cell containing the lipophilic bioactive substance or a microbial cell homogenate thereof and an organic solvent in the presence of a particular surfactant, and extracting the lipophilic bioactive substance into the organic solvent phase. This production method enables extraction without using special dehydrating, drying facility, and without causing a decrease in the yield due to degraded separability between solvent and fungus body component, as well as efficient industrial production.

Method for manufacturing a fat-soluble bioactive substance

A method for production of fine microcapsules which encapsulate a hydrophilic bioactive substance at a high content and can be used in wide range of applications such as foods and medical drugs, which method enabling efficient industrial production, is provided. A method for production of S/O type microcapsules including the steps of: (1) emulsifying and dispersing a mixture of a solid fat and an aqueous solution containing a hydrophilic bioactive substance at a temperature of at least the melting point of the solid fat to obtain a W/O emulsion; (2) removing moisture in the W/O emulsion at a temperature of at least the melting point and lower than the boiling point of the solid fat to obtain an S/O suspension; (3) adding the S/O suspension into an aqueous phase containing at least one selected from a surfactant (B), a thickening agent and a hydrophilic organic solvent, and permitting liquid droplet dispersion at a temperature of at least the melting point and lower than the boiling point of the solid fat to obtain an S/O/W emulsion; and (4) cooling the S/O/W emulsion to lower than the melting point of the solid fat to harden the solid fat, and further removing the moisture at a temperature lower than the melting point of the solid fat.

Method for production of microcapsules using solid fat

The present invention relates to a process for producing reduced coenzyme Qwhich comprises obtaining microbial cells containing reduced coenzyme Qat a ratio of not less than 70 mole % among the entire coenzymes Q, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q. The present invention also relates to a process for producing oxidized coenzyme Qwhich comprises either recovering oxidized coenzyme Qafter oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Qfrom the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Qthereafter. According to the processes of the present invention, reduced coenzyme Qand oxidized coenzyme Qcan be produced simply on the industrial scale.

Growing community of inventors

Osaka, Japan

Akihisa Kanda