Hitachi-high-technologies Corporation

Akihiro Takeda

Kinya Kobayashi

Tsukasa Shishika

Hiromichi Yamashita

Shinji Yoshioka

Tomoyuki Kurosawa

Provided is a mass spectroscope employing electron capture dissociation wherein the peak number of detectable fragment ions is increased. The mass spectroscope comprises an ion source () for generating ions from a sample, an ion trap () for storing and selecting ions, an ion dissociation section () performing electron capture dissociation on ions, and a time-of-flight mass spectrometry section () performing mass spectrometry on ions, wherein the reaction time of electron capture dissociation is variable depending on the valence of ions subjected to mass spectrometry.

Mass spectroscope and mass spectrometry

The present invention achieves a mass analysis method that can identify protein or peptide with high speed and high sensitivity. A mass spectrum is obtained from a standard sample of healthy person, an ion is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(a) to (d)]. A mass spectrum is obtained from a sample of interest to be measured of patient, an ion other than the precursor ion of the standard sample is selected from the mass spectrum as a precursor ion, and a mass spectrum of the precursor ion is obtained [(g) to (k)]. Identifications on peptide/protein specific to the standard sample and the sample to be measured, and common to both are conducted [(r) to (q)], and based on the results, comparative analysis on peptide/protein of the sample to be measured (t). Without regarding an ion derived from all components of the sample to be measured as precursor ion, MS/MS spectrum is obtained and identification on a plurality of components of the sample to be measured can be conducted for a short time and with high sensitivity.

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Hitachinaka, Japan

Akihiro Takeda