Bonac Corporation

Nippon Steel & Sumikin Engineering Co., Ltd.

Sumitomo Chemical Company, Limited

Akihiro Itoh

Hiroshi Suzuki

Tadaaki Ohgi

Tomohiro Hamasaki

Hisao Shirohzu

Hirotake Hayashi

Eriko Aoki

Kazuhiro Takahashi

Hideki Fujii

Akira Kawakami

Teruhiko Hayashi

Takashi Kinoshita

Masayuki Igarashi

Keita Suzumura

Masahiro Hayashi

Kazuhiro Harasaka

Shunichi Sato

Katsunari Hanaoka

The invention provides a production method of a glycoside compound or a salt thereof, which includes subjecting a thioether compound and an alcohol compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid, and then distillation in the presence of at least one kind of additive selected from a sulfur-containing antioxidant and a maleimide group-containing compound to give a thioether compound and a step of subjecting a glycoside compound and a thioether compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid to give a glycoside compound. By this method, a phosphoramidite preferable for the production (synthesis) of a nucleic acid can be produced more efficiently at a high purity.

Method for producing glycoside compounds

The invention provides a glucoside compound, which is capable of providing a phosphoramidite, which can be produced at low cost and can produce a nucleic acid in high yield and with high purity. The glycoside compound has the formula wherein B, R1, R2, and R3 are as described herein.

Glycoside compound, method for producing thioether, ether, method for producing ether, method for producing glycoside compound, method for producing nucleic acid

The invention provides a glucoside compound, which is capable of providing a phosphoramidite, which can be produced at low cost and can produce a nucleic acid in high yield and with high purity. The glycoside compound has the formula wherein B, R, and Rare as described herein, Ris &#x2014;OCH(OCH)&#x2014;O-L-[D], in which Lis an ethylene group, wherein hydrogen atoms besides a hydrogen atom bonded to the &#x3b1;-position relative to [D] are optionally substituted by a straight chain or branched alkyl group, n is a positive integer, and [D] is an electron-withdrawing group.

Provided is a multiplex pipe and a system for recovering steam from a geothermal well, which are capable of solving all of the various problems with a neutralization pipe prepared by painting or the like of an alkali-resistant coating on the inner face of an acid-resistant single pipe. A multiplex pipethat is configured to recover steam from a geothermal well GT, includes: a casing pipelocated outside; and a duplex pipethat is slidable relative to the casing pipein the casing pipe, and has a first gapwith an inner face of the casing pipe, the duplex pipe including an outer pipehaving acid-resistance and an inner pipehaving alkali-resistance, the outer pipeand the inner pipedefining a second gaptherebetween. An alkali solution is injected to acid hot-water stored in an acid geothermal well GT via a hollowof the inner pipeto neutralize the acid hot-water, and the neutralized hot-water V is allowed to spout to the ground via the first gap, thus recovering flashing steam.

Multiplex pipe and system for recovering steam from geothermal wells

Provided is a novel nucleic acid molecule that can be produced easily and efficiently and can inhibit the expression of a gene. The nucleic acid molecule is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes: a region (X); a linker region (Lx); and a region (Xc). The linker region (Lx) is linked between the regions (Xc) and (Xc). The region (Xc) is complementary to the region (X). At least one of the regions (X) and (Xc) includes the expression inhibitory sequence. The linker region (Lx) has a non-nucleotide structure including at least one of a pyrrolidine skeleton and a piperidine skeleton. According to this single-stranded nucleic acid molecule, it is possible to inhibit the expression of the target gene.

Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton

Provided is a novel nucleic acid molecule that is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes, in sequence from the 5' side to the 3&#x2032; side: a 5&#x2032; side region (Xc); an inner region (Z); and a 3&#x2032; side region (Yc). The inner region (Z) is composed of an inner 5&#x2032; side region (X) and an inner 3&#x2032; side region (Y) that are linked to each other. The 5&#x2032; side region (Xc) is complementary to the inner 5&#x2032; side region (X). The 3&#x2032; side region (Yc) is complementary to the inner 3&#x2032; side region (Y). At least one of the inner region (Z), the 5&#x2032; side region (Xc), and the 3&#x2032; side region (Yc) includes the expression inhibitory sequence.

Single-stranded nucleic acid molecule for controlling gene expression

Growing community of inventors

Kurume, Japan

Akihiro Itoh