Fujifilm Corporation

Akihiro Asakura

Kousuke Watanabe

Yoshinori Kanazawa

Sho Onozawa

Kazuhei Kaneko

Sohichiro Nakamura

Hiyoku Nakata

Ryusuke Osaki

Keiko Makita

Provided are any one of the following fluorescent compounds and a fluorescent labeled biological substance having this fluorescent compound. However, in a case where the ring βand the ring βare a 6-membered ring, and Land Lare an arylene group, Ris not an aryl group substituted with a linear alkyl group having 18 or more carbon atoms. In addition to the regulation of the ring β, the ring β, L, and L, in a case where Rhas a substituent having a dipyrromethene boron complex structure, the dipyrromethene boron complex structure has a structure in which a dipyrromethene skeleton is coordinately bonded to a boron atom in the tridentate or tetradentate coordination.

Fluorescent compound and fluorescent labeled biological substance using the same

Provided is a determination method capable of non-destructively and simply determining a state of a sphere that is an aggregate of a plurality of cells. A phase difference image of a sphere that is an aggregate of a plurality of cells is generated from a hologram obtained by imaging the sphere, and a state of the sphere is determined on the basis of the phase difference image and a shape index value corresponding to a shape of the sphere.

Method for determining a state of a sphere

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Kanagawa, Japan

Akihiro Asakura