Hamamatsu-photonics K.k.

Laboratory of Molecular Biophotonics

Akihiko Tsuji

Kaname Ishibashi

Tadashi Fukami

Shigeru Hosoi

Hiroyuki Koshimoto

Yoshihiro Sato

Satoshi Kondo

Takayuki Suga

Yukari Iida

Makiko Hiyoshi

Masahiko Hirano

The present invention provides a method for selectively separating live cells which have expressed a specific mRNA comprising: a first step of introducing a marker which label mRNA into cells in a live cell group containing live cells which have expressed a specific mRNA; a second step of labeling said mRNA with said marker to obtain a live cell group containing live cells having the labeled mRNA; and a third step of detecting said labeled mRNA to identify the live cells having the labeled mRNA and separating the identified live cells selectively from said live cell group obtained in said second step.

Method for selectively separating live cells expressing a specific gene

Detection probes and a method of detection for the detection of a specimen having a specified sequence in a sample are disclosed that utilize two fluorophore-labeled (donor and acceptor) probes which are designed to display changes in a fluorescence decay curve by their hybridization to the specific sequence of a specimen, thus allowing for the detection with great accuracy and high sensitivity, particularly under the conditions where the probes may be abundant relative to the specimen in the sample.

Probes and methods for polynucleotide detection

Detection probes labeled with fluorescent dyes, to which are bound nuclear membrane unpermeable molecules via linkers, having base sequences that can hybridize to a target nucleic acid. The probes are introduced into the cytoplasm of a living cell in which the target nucleic acid is present, and the target nucleic acid is detected by measurement of the change in fluorescence of the fluorescent dyes due to the formation of a hybrid of the target nucleic acid and the probes.

Nucleic acid detection in cytoplasm

This invention provides a method for monitoring the transcriptional synthesis of RNA, and to an apparatus therefor. Specifically, the invention resides in monitoring the initiation and termination of a transcription reaction for RNA synthesis, as well as the synthesis of full-length RNA by measuring the fluorescence of a pair of oligonucleotide probes of two types having a base sequence that continuously hybridizes to a part of a base sequence of the RNA synthesized by transcription, the pair of oligonucleotide probes comprising a donor probe labeled with an energy donor fluorescent molecule and an acceptor probe labeled with an energy acceptor fluorescent molecule.

Method for monitoring transcriptional synthesis of RNA

A plurality of light emission molecules, having different light emission lifetimes, are irradiated with excitation light so that the light emission molecules emit light. The emitted light is divided into at least two different wavelength regions. Temporal changes in each of the at least two wavelength regions of the light emission is measured over at least two different time periods. Information on energy transfer generated between the plurality of light emission molecules is determined based on the measured results in each of the wavelength regions over each of the time periods.

Instrument for measuring fluorescence resonance energy transfer

An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling means for controlling condition of denaturation under which a double-stranded nucleic acid is separated into a first single-stranded nucleic acid and a second single-stranded nucleic acid; excitation light irradiation means for irradiating the double-stranded nucleic acid before denaturation, and the first single-stranded nucleic acid and second single-stranded nucleic acid after the denaturation; fluorescence detection means for detecting fluorescence emission based on the excitation light irradiation; and processing means for receiving, storing and processing a signal supplied from the fluorescence detection means.

Method and apparatus for detecting denaturation of nucleic acid

Method for measuring fluorescence resonance energy transfer

An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling element for controlling condition of denaturation under which a double-strand nucleic acid is separated into a first single-strand nucleic acid and a second single-strand nucleic acid; excitation light irradiation source for irradiating the double-strand nucleic acid before denaturation, and the first single-strand nucleic acid and second single-strand nucleic acid after the denaturation; fluorescence detection monitor for detecting fluorescence emission based on the excitation light irradiation; and processing element for receiving, storing and processing a signal supplied from the fluorescence detection monitor.

Growing community of inventors

Shizuoka, Japan

Akihiko Tsuji