Kobe University

Kansai Chemical Engineering Co., Ltd.

Bio-energy Corporation

Dic Corporation

Inter-university Research Institute Corporation National Institutes of Natural Sciences

Beacle, Inc.

Toyota Jidosha Kabushiki Kaisha

Kaneka Corporation

Nippon Shokubai Co., Ltd.

Japan Science and Technology Agency

Kabushiki Kaisha Toyota Chuo Kenkyusho

Jnc Corporation

Akihiko Kondo

Hideo Noda

Keiji Nishida

Tomohisa Hasunuma

Hideki Fukuda

Jo-Shu Chang

Masakazu Ueda

Tsutomu Tanaka

Masaharu Seno

Chiaki Ogino

Katsuyuki Tanizawa

Jun Ishii

Haruo Nishie

Satomi Kojima

Shih-hsin Ho

Jun Minagawa

Hiroyuki Taroda

Noriyuki Ohnishi

Takeshi Matsumoto

Yasuyuki Nakamura

Satoshi Katahira

Nobuhiro Ishida

Haruo Takahashi

Hirotaka Furukawa

Shinji Hama

Teruyuki Nishi

Kageaki Matsui

Eita Ichige

Nobuyuki Kuratani

Shunichi Kuroda

Masaharu Mukoyama

Kazuhide Tabata

Risa Nakamura

Hideyuki Hata

Kenji Tsuge

Yoichiro Ito

Takayuki Arazoe

Shin Yoshioka

Shun'ichi Kuroda

Toshihide Okajima

Kazunori Nakashima

Ryosuke Yamada

Shohei Kaneko

Ryohei Sasaki

Kenji Okano

Hideo Nishizawa

Takako Nogami

Yuri Sakihama

Sung-Jin Jo

Kentaro Inokuma

Zenpei Shimatani

Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted DNA using cell endogenous DNA modifying enzyme, and molecular complex used therein

The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Genomic sequence modification method for specifically converting nucleic acid bases of targeted DNA sequence, and molecular complex for use in same

The present invention addresses the problem of providing a novel method which is for preparing a DNA fragment for microbial cell transformation, and by which the combinatorial library of a long-chain DNA can be efficiently constructed and confirmation of the genotype of the obtained clone is facilitated. The present invention is a method for preparing a DNA fragment, which is for microbial cell transformation and has at least one insert DNA unit that includes a DNA containing an effective replication origin in a host microorganism and an insert DNA in which unit DNAs are linked, the method being characterized by including: (A) a step for preparing, through an OGAB method, a plurality of types of plasmids having an insert DNA unit in which a plurality of types of unit DNAs capable of being linked in a specific linking order are linked; (B) a step for decomposing a plasmid into unit DNAs by treating the plurality of types of plasmids prepared in the step (A) with a restriction enzyme suitable for each plasmid and preparing a mixed liquid of a plurality of types of unit DNAs; and (C) a step for preparing a long-chain DNA fragment by re-assembling the unit DNAs through the OGAB method by using the mixed liquid of a plurality of types of unit DNAs obtained in the step (B).

Method for constructing chimeric plasmid library

One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.

Cell and method for producing target protein using same

The present invention provides a method of modifying a targeted site of a double stranded DNA of a monocot cell, comprising a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the given double stranded DNA and a nucleic acid base converting enzyme are bonded, with said double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into said targeted site, without cleaving at least one strand of said double stranded DNA in the targeted site, wherein the double stranded DNA is contacted with the complex by introducing a nucleic acid encoding the complex into the monocot cell. Furthermore, also provided is a complex used for the method, wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a monocot cell and a nucleic acid base converting enzyme are bonded.

Method for converting monocot plant genome sequence in which nucleic acid base in targeted DNA sequence is specifically converted, and molecular complex used therein

The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Method for modifying genome sequence to introduce specific mutation to targeted DNA sequence by base-removal reaction, and molecular complex used therein

The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.

Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same

There is provided a method for generating an oil/fat component, in which salt-tolerant algae are cultured in a culture medium of which the salt concentration has been increased in a stepwise manner.

Method for producing an oil or fat component and method for producing higher unsaturated fatty acid using algae

There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging toare cultured in a culture medium containing sea salt.

Method for generating oil/fat component from chlamydomonas algae

Disclosed is a polynucleotide for cell surface expression. The polynucleotide of the invention comprises a promoter, a secretion signal sequence, a sequence encoding an intended protein, and a sequence encoding a cell surface-localized protein or a cell membrane-binding domain thereof, wherein the promoter is a promoter of a gene encoding the cell surface-localized protein. Provided is a polynucleotide for cell surface expression allowing the production of yeast displaying an enzyme on the cell surface with a high activity.

Polynucleotide for cell surface layer expression

The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.

Method for producing useful substance from cellulose-containing material

A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.

Method for producing ethanol

Provided is a method for efficiently producing ethanol by ethanol fermentation from xylose using a saccharified biomass, which contains various fermentation inhibitors. A method for producing ethanol from biomass of the present invention includes the step of culturing a xylose-utilizing yeast transformed so as to overexpress a gene for an acetic acid-responsive transcription factor in combination with a saccharified biomass.

Method for producing ethanol from biomass

The present invention provides a method for measuring oocyst of protozoa, such as, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein. Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.

Method for measuring protozoan oocyst and detecting reagent

Growing community of inventors

Kobe, Japan

Akihiko Kondo