Takara Bio Inc.

Takara Shuzo Co., Inc.

Akihiko Kita

Ikunoshin Kato

Takashi Kawai

Katsuhiko Kusakabe

Jun Tomono

Susumu Tsunasawa

Hiroko Ohta

The present invention provides a fungal bed culture of a hon-shimeji mushroom inoculated with a liquid seed culture wherein the surface of a culture medium for cultivation has a liquid seed culture inoculated portion and a liquid seed culture non-inoculated portion as well as provides a fungal bed cultivation method of a hon-shimeji mushroom which generates a fruit body from the fungal bed culture. According to the present invention, the formation rate of budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved, thereby enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation.

Hon-shimeji mushroom-fungal bed culture

Problem: To provide a method for fungal bed cultivation of a mushroom of a large size having an excellent shape and crunchy texture, a mushroom cutting useful for the fungal bed cultivation method, a culture medium for fungal bed cultivation into which the cutting is transplanted, and a culture medium suitable for fungal bed cultivation. Solution: A method for cultivating a mushroom in a fungal bed comprising a step of transplanting an isolated cutting of the mushroom into a culture medium for fungal bed cultivation, an isolated cutting of a mushroom to be used in the method, and a culture medium for fungal bed cultivation of a mushroom into which the cutting is transplanted.

Method for fungal bed cultivation of mushroom

According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such.

Method for cultivating hon-shimeji mushroom on fungal bed

A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.

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Shiga, Japan

Akihiko Kita