Panasonic Healthcare Co., Ltd.

Sanyo Electric Co., Ltd.

Panasonic Healthcare Holdings Co., Ltd.

Phc Holdings Corporation

Shibuya Kogyo Co., Ltd.

Phc Corporation

Akifumi Iwama

Yasuhiko Yokoi

Jiro Ohnishi

Masaki Harada

Shinji Fukui

Hiroshi Yamamoto

Yoshiaki Noguchi

Masahiro Iseki

Azusa Nakagawa

Yuichi Tamaoki

Hironobu Sekine

Atsushi Nakao

Jirou Ohnishi

Yukihiro Sugiyama

Kanjun Yamamoto

Tatsuya Hirose

Hironobu Sunayama

Yasuaki Sonoda

Yoshiaki Sugimura

Katsuya Hirai

Eiji Kono

There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wellsA are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each wellA. A fluorescence measurement value [DNA]raw obtained from the wellA and a fluorescence measurement value [DNA]bg obtained from a connection wall near the wellA are detected, and the fluorescence measurement value [DNA]bg is subtracted from the fluorescence measurement value [DNA]raw to determine fluorescence strength [DNA]real of the wellA.

Method for detecting nucleic acid amplification product in real time

An isolator includes a work chamber, a sterilizing substance supply unit, a gas flow channel pressure adjustment unit, a work chamber barometer, and a controller. The controller is configured to control execution of a gas flow channel leak test for checking a gas leak in a gas flow channel based on a detection result by the work chamber barometer after making the gas flow channel pressure adjustment unit increase or decrease the pressure in the gas flow channel, and is configured to control supply of the sterilizing substance by the sterilizing substance supply unit. The controller performs heating of a heater, which accompanies the supply of the sterilizing substance, in parallel with the gas flow channel leak test.

Isolator

An isolator for cultivating cells including a working chamber having a plurality of gloves arranged side by side into which operator's hands are inserted to operate cells, the working chamber being sectioned into at least an operation area for operating the cells, and an auxiliary working area for opening a packaged auxiliary instrument used to operate the cells, a gas supply unit that supplies gas into the working chamber so that the gas flows downwardly from an upper side in the working chamber, and a gas flow control unit for controlling the flow of the downwardly flowing gas so that the gas flows from the operation area to the auxiliary working area around the gloves, wherein the gas flow control unit has an exhaust hole portion that has an opened area for passing the gas therethrough and is provided at a lower portion of at least the auxiliary working area, and through which the gas in the auxiliary working area is exhausted.

Isolator for cell culture

A sterile-substance-supplying apparatus comprising: an atomization unit including a reservoir unit to reserve hydrogen peroxide, and an ultrasound vibrator to impart ultrasound vibration to the reserved hydrogen peroxide to be atomized; an evaporation unit, provided above the reservoir unit, that heats the atomized hydrogen peroxide to be evaporated; a connecting member connecting the evaporation unit and the atomization unit to each other; a hydrogen-peroxide-supply port to allow the hydrogen peroxide to be supplied to the reservoir unit; a carrier-gas-supply port, provided at the connecting member, that allows carrier gas for allowing the atomized hydrogen peroxide to flow to the evaporation unit to be supplied; and a container portion to reserve a substance for transmitting the ultrasound vibration generated by the ultrasound vibrator, and a metal vibrating plate, provided at a bottom portion of the reservoir unit, that transmits to the hydrogen peroxide the ultrasound vibration transmitted through the substance.

Sterile substance supplying apparatus

An incubator including a cultivating chamber for cultivating cultures, a water supply control unit for supplying water into the cultivating chamber through a water supply passage and adjusting the humidity of the inside of the cultivating chamber, and a filter provided in the water supply passage.

Incubator

An isolator includes a workroom having a first sterilizing material supply port, a first sterilizing material discharge port, a first gas supply port and a first gas discharge port, a sterilization chamber having a second sterilizing material supply port, a second sterilizing material discharge port, a second gas supply port and a second gas discharge port, a sterilizing material supply unit, a first sterilizing material supply path connecting the sterilizing material supply unit to the first sterilizing material supply port, a second sterilizing material supply path connecting the sterilizing material supply unit to the second sterilizing material supply port, a first circulating path connecting the first sterilizing material discharge port to the sterilizing material supply unit, a second circulating path connecting the second sterilizing material discharge port to the sterilizing material supply unit, a first gas supply path, a second gas supply path, a first gas discharge path, a second gas discharge path, and a control unit.

A sterilizer is connectable to an external chamber. A sterilization gas generator is configured to generate sterilization gas. A gas supply system including a first gas supply system which is configured to supply the sterilization gas from the sterilization gas generator to a sterilization chamber; and a second gas supply system which is different from the first gas supply system and configured to supply the sterilization gas from the sterilization gas generator to the external chamber.

Sterilizer

A sterilizing material supply apparatus has a sterilizing material gas generator including a mist generation unit and a vaporizing unit. In the mist generation unit, hydrogen peroxide solution in a cup is converted into a mist by ultrasonic vibration generated by an ultrasonic vibrator. The hydrogen peroxide solution which has been turned into the mist is heated by a heater in the vaporizing unit so as to be gasified. The gasified hydrogen peroxide solution is supplied to a workroom of an isolator as a sterilizing gas.

Sterilizing material supply apparatus and isolator

A method for detecting and identifying a chemical substance in various phases such as a vapor phase and a liquid phase. The chemical substance being measured is diffused into a predetermined phase. Then, an electric state at an electrode existing in the predetermined phase is detected as a change with time based on the diffusion and absorption of the chemical substance on the electrode. A pattern of the change of the electric state with time is prepared. A fitting function based on the pattern is set and parameters of the chemical substance being measured are found. A distance between the parameters of the chemical substance being measured and parameters of the reference substance which is detected is determined. In the case of a detection of a chemical substance in a liquid phase, before detecting the electric state at the electrode existing in the liquid phase, a predetermined electrolytic solution is supplied around the electrode to stabilize the electric state of the electrode and then the liquid phase containing the chemical substance being measured is supplied to the electrode.

Method for detecting chemical substances

On the bottom face of a measurement section, measurement electrodes and a reference electrode are formed and a solution supply pipe is opened. Then, if a predetermined electrolytic solution is supplied from the solution supply pipe, the electrolytic solution can be held on an electrolytic solution holding surface on the lower end of the measurement section, utilizing the with surface tension thereof for providing a gas reception portion. Since the electrolytic solution is exposed directly to the air, substances in a vapor phase (particularly, odor substances) are diffused into the electrolytic solution, causing an electric state to change. The substances in the vapor phase can be detected by detecting the electric state change. The electrolytic solution held on the lower face of the measurement section can be updated by further supplying more electrolytic solution.

Vapor phase sensor

On the bottom face of a measurement section, measurement electrodes and a reference electrode are formed and a solution supply pipe is opened. Then, if a predetermined electrolytic solution is supplied from the solution supply pipe, the electrolytic solution can be held on an electrolytic solution holding surface on the lower end of the measurement section, utilizing the surface tension thereof for providing a gas reception portion. Since the electrolytic solution is exposed directly to the air, substances in a vapor phase (particularly, odor substances) are diffused into the electrolytic solution, causing an electric state to change. The substances in the vapor phase can be detected by detecting the electric state change. The electrolytic solution held on the lower face of the measurement section can be updated by further supplying more electrolytic solution.

Growing community of inventors

Tsukuba, Japan

Akifumi Iwama