Seiko Epson Corporation

Akemi Yamaguchi

Hiroshi Koeda

A thermal cycler includes an attachment unit having an insertion opening for insertion of a reaction container including a channel filled with a reaction solution containing reverse transcriptase enzyme and a liquid having a lower specific gravity than that of the reaction solution and being immiscible with the reaction solution, a first heating unit that heats a first region of the channel, a second heating unit that heats a second region of the channel nearer the insertion opening than the first region, a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement, and a control unit that controls the first heating unit to be at a temperature at which reverse transcription reaction progresses and the second heating unit to be at a temperature at which the reverse transcriptase enzyme is not deactivated.

Thermal cycler and control method of thermal cycler

An attachment unit for attachment of a reaction container including a channel filled with a reaction solution containing a fluorescent probe and a liquid having a specific gravity different from that of the reaction solution and being immiscible with the reaction solution, the reaction solution moving close to opposed inner walls, a first heating unit heating a first region of the channel and a second heating unit heating a second region of the channel when the reaction container is attached to the attachment unit, a drive mechanism switching arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement in which a lowermost position of the channel is located within a first region and a second region, respectively, a measurement unit measuring light intensity, and a control unit controlling the portions described above.

An attachment unit for attachment of a reaction container including a channel filled with a reaction solution and a liquid having a specific gravity different from that of the reaction solution and being immiscible with the reaction solution, the reaction solution moving close to opposed inner walls, a first heating unit that heats a first region of the channel and a second heating unit that heats a second region of the channel when the reaction container is attached to the attachment unit, a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement in which a lowermost position of the channel is located within a first region and a second region, respectively, and a control unit that controls the drive mechanism, the first heating unit, and the second heating unit are provided.

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Matsumoto, Japan

Akemi Yamaguchi