Simpore Inc.

Akash S Patel

James A Roussie

Jared A Carter

Cassandra R Walinski

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

Extracellular vesicle isolation by nanomembranes

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereb forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

Growing community of inventors

Utica, NY, United States of America

Akash S Patel