Pioneer Hi-Bred International, Inc.

E.i. Dupont De Nemours and Company

Ajith Anand

William James Gordon-Kamm

Keith S Lowe

Theodore Mitchell Klein

Ning Wang

George J Hoerster

Shiv Bahadur Tiwari

Alexandre Da Silva Conceição

Xinli Emily Wu

Carlos M La Rota

Maren L Arling

Anthony John Kinney

Michael W Lassner

Kevin E McBride

Sandeep Kumar

Steven Henry Bass

Jun-Zhi Wei

Sean M Bertain

Zhan-bin Liu

Craig E Hastings

Jun-zhi Wei

Todd J Jones

Hyeon-Je Cho

York Moy

Barbara Ann Marie Rosen

Sergi Svitashev

Kevin E Mcbride

Barabara Ann Marie Rosen

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Methods and compositions for rapid plant transformation

Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.

Methods and compositions for homology directed repair of double strand breaks in plant cell genomes

Methods and compositions for-mediated transformation of plants are provided. Methods include but are not limited to using anstrain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include anstrain, transfer DNAs, constructs and/or plasmids. These includestrains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

Ochrobactrum-mediated transformation of plants

Growing community of inventors

West Des Moines, IA, United States of America

Ajith Anand