Medimmune Limited

Memimmune, LLC

Ajit Subramanian

Richard Schwartz

John Michael Berry

Xiao Shi

Jonathan Edward Liu

Mark Thompson

Mridul Ghosh

Simon Sheng-Tsiung Hsu

Luis J Maranga

Luis Jorge Camilo Maranga

Luis Maranga

Simon Hsu

The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.

MDCK cell lines supporting viral growth to high titers and bioreactor process using the same

The present invention provides novel MDCK-derived adherent non-tumorigenic cell lines that can be grown in the presence or absence of serum. The cell lines of the present invention are useful for the production of vaccine material (e.g., viruses). More specifically, the cell lines of the present invention are useful for the production of influenza viruses in general and ca/ts influenza viruses in particular. The invention further provides methods and media formulations for the adaptation and cultivation of MDCK cells such that they remain non-tumorigenic. Additionally, the present invention provides methods for the production of vaccine material (e.g., influenza virus) in the novel cell lines of the invention.

Non-tumorigenic MDCK cell line for propagating viruses

Method of purifying influenza virus and removing MDCK cell DNA contaminants

Growing community of inventors

Berkeley, CA, United States of America

Ajit Subramanian