Nanjing Bestzyme Bio-engineering Co., Ltd.

Aixi Bai

Hong Xu

Jidong Zhu

Feng Li

Yan Sun

Fei Zheng

Furong Bian

Ting Yan

Disclosed is a thermostable phytase, in which at least one pair of introduced disulfide bonds is included in the amino acid sequence of wild-typephytase or mutantphytase, and after the introduction, the properties of the phytase can be improved, especially the heat stability, steam stability and granulation stability, which are superior to those of the existing wild-type or mutant phytase; compared to the engineered phytase in which disulfide bonds are introduced, the heat stability is also significantly improved.

Phytase mutant

Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-typeglucose oxidase or a mutantglucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.

Thermostable glucose oxidase

Disclosed herein is a method for expressing phytase in a filamentous fungus by using an optimizedphytase gene having a nucleotide sequence as shown in SEQ ID NO. 7 and a signal peptide having a nucleotide sequence as shown in SEQ ID NO. 12.

Expression of phytase in

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Jiangsu, China

Aixi Bai