Genentech, Inc.

William Marsh Rice University

Ailen M Sanchez

George Nelson Bennett

Martin Vanderlaan

X Christopher Yu

Susan C Fisher

Ka-Yiu San

Charles H Schmelzer

Shelly Pizarro

John B Lowe

Atia Naim

Jayme Franklin

Christopher A Teske

San Ka-Yiu

Corazon Victa

Saloumeh Kadkhodayan Fischer

Chandresh Thakker

Jiangfeng Zhu

Grant Balzer

Annamarie Amurao

Cheryl Renee Dittrich

John Lowe

Purified recombinant polypeptides isolated from Chinese hamster ovary host cells, including antibodies, such as therapeutic antibodies, and methods of making and using such polypeptides are provided.

Methods and compositions comprising purified recombinant polypeptides

Compositions comprising purified recombinant IL-13 antibody

Method of treatment comprising purified recombinant IL-13 antibody

Processes are provided for recovering and purifying refolded recombinant proteins produced in heterologous host cells, which includes the step of refolding the protein in a high pH buffer.

Refolding of recombinant proteins

Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are &#x394;adhE&#x394;ldhA&#x394;iclR&#x394;ack-pta plus PYCand NAD-dependant FDH.

Increasing bacterial succinate productivity

The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.

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In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

Simultaneous anaerobic production of isoamyl acetate and succinic acid

A method of increasing cellular NADPH levels by expressing one or more genes that encode a enzyme that causes the production of NADPH. The system can be combined with other enzymes that require NADPH, thus improving the overall production of the desired protein.

Growing community of inventors

Foster City, CA, United States of America

Ailen M Sanchez