Beijing Dabeinong Biotechnology Co., Ltd.

Beijing Dabeinong Technology Group Co., Ltd

Beijing Dabeinong Technology Group Co., Ltd., Biotech Center

Beijing Green Agrosino Plant Protection Technology Co., Ltd

Aihong Zhang

Derong Ding

Qing Tao

Xiaojiao Li

Jie Pang

Ruiqi Niu

Lihong Niu

Chao Gao

Na Wang

Jincun Huang

Yujie Qi

Mengmeng Miao

Donghai Yang

An isolated polynucleotide includes: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence comprising at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, wherein the ingestion, byinsect pests, of a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence inhibits the growth of theinsect pests; or (c) any one of the polynucleotide sequence as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a), (b) or (c) mentioned above under stringent conditions. Multiple target sequences of the target gene c46312 control theinsect pest

Polynucleotide and method for controlling insect infestation

An isolated polynucleotide includes: (a) a polynucleotide sequence as shown in SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15, 17, 19 or 21 contiguous nucleotides of SEQ ID NO: 1, wherein the growth of a pest of the order Coleoptera is inhibited when the pest of the order Coleoptera ingests double-stranded RNA comprising at least one strand that is complementary to the described polynucleotide sequence; or (c) any polynucleotide sequence as shown in SEQ ID NO: 3 to SEQ ID NO: 6; or (d) a polynucleotide sequence which hybridizes under stringent conditions with the polynucleotide sequence as defined in (a), (b) or (c). A plurality of target sequences are used for controlling a target gene c4506 of, which is a pest of the order Coleoptera.

Polynucleotide and method used for controlling insect invasion

Provided are an isolated polynucleotide and a method for controlling insect invasion. The isolated polynucleotide is a plurality of target sequences for controlling target gene c35112 of a coleopteran pest,, comprising: a) a polynucleotide sequence shown as SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence being capable of inhibiting the growth of coleopteran pests after being ingested by the coleopteran pests; or (c) any one of polynucleotide sequences shown as SEQ ID NO: 3 to SEQ ID NO: 20; or (d) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence as defined in (a), (b) or (c).

Polynucleotide and method for controlling insect invasion

Disclosed are a nucleotide sequence and a method for controlling insect invasions, the isolated polynucleotide sequence comprising: (a) a polynucleotide sequence as shown in SEQ ID NO: 1, 2 or 3; or (b) a polynucleotide sequence hybridizing with the polynucleotide sequence defined in (a) under stringent conditions; or (c) a polynucleotide sequence having an identity of not less than 80% to the polynucleotide sequence defined in (a); or (d) a polynucleotide sequence of at least 19 consecutive nucleotides of the polynucleotide sequence defined in (a), wherein a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence, when ingested by an insect pest from, inhibits growth of the insect pest; or (e) a complementary sequence of the polynucleotide sequence as defined in (a), (b), (c) or (d). The three target sequences for controlling an insect pest from, have the advantages of efficiency, specificity, convenience and low cost.

Nucleotide sequence and method for controlling insect infestation

The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2; or (b) a protein derived from (a) consisting of an amino acid sequence by substitution, deletion, or addition of one or more amino acid residues of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a nucleotide sequence of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with SEQ ID NO:1 under stringent conditions; or (e) a protein generated by the expression of nucleic acid molecules that contain nucleotide sequences isocoding with the nucleotide sequences in (d). The insecticidal protein of the invention has high expression level and strong toxicity against pests.

Insecticidal protein, gene encoding the same and uses thereof

The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2 or a sequence of amino acid residuses at positions 1 to 640 thereof; or (b) a protein derived from (a) by substitution, deletion, or addition of one or more amino acid residuses of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a sequence of amino acid residuses at the positions 1 to 1920 of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with the sequence of nucleotides at the positions 1 to 1920 of SEQ ID NO:1 under stringent conditions. The protein has high expression level and strong toxicity against pests.

Growing community of inventors

Beijing, China

Aihong Zhang