Commonwealth Scientific and Industrial Research Organisation

Arista Cereal Technologies Pty Limited

Limagrain Cereales Ingredients S.a.

Biogemma S.a.s.

Arista Cereal Technology Pty Limited

Limagrain Céréales Ingrédients

Ahmed Regina

Matthew Kennedy Morell

Sadequr Rahman

Bernard Duperrier

Elisabeth Marie-Anne Ida Chanliaud

Pierre Georges Louis Berbezy

Zhongyi Li

David Lloyd Topping

Anthony Richard Bird

Gulay Saygat Mann

Charles James Peterson

Provided are food and drink ingredients produced from wheat grain which has a low level (2-30%) of total starch branching enzyme II activity and an amylose content in the starch of at least 50% (w/w), and processes for producing and using the ingredients. Also provided are foods produced from the ingredients which may be used in humans to improve one or more parameters of metabolic health, bowel health or cardiovascular health.

Food ingredients produced from high amylose wheat

Wheat grain () comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.

High amylose wheat with undetectable levels of SBEIIa protein

High amylose wheat&#x2014;II

A wheat variety designated LSPA15-HD253, the plants and seeds of wheat variety LSPA15-HD253, methods for producing a wheat plant produced by crossing the variety LSPA15-HD253 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety LSPA15-HD253 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety LSPA15-HD253 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety LSPA15-HD253 and to wheat varieties or breeding lines produced by those methods are also provided.

Wheat variety LSPA15-HD253

A wheat variety designated LSPA15-HD307, the plants and seeds of wheat variety LSPA15-HD307, methods for producing a wheat plant produced by crossing the variety LSPA15-HD307 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety LSPA15-HD307 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety LSPA15-HD307 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety LSPA15-HD307 and to wheat varieties or breeding lines produced by those methods are also provided.

Wheat variety LSPA15-HD307

High amylose wheat-II

Provided is a Wheat grain () comprising an embryo, starch and one, two or three SBEIIa proteins, said embryo comprising two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein the starch has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain, and wherein at least one of the SBEIIa proteins is produced in the developing wheat endosperm and has starch branching enzyme activity.

High amylose wheat

Wheat having a reduced level of SBEIIa activity, that may have a relative high amylose content. Wheat having a mutant SBEIIa gene in the A genome. The wheat might additionally have reduced levels of SBEIIb activity. The wheat grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway, and may also have a high relative amylose content.

Wheat with altered branching enzyme activity and starch containing products derived therefrom

A method and composition for improving one or more indicators of bowel health or metabolic health in a mammalian animal. This comprises the delivering to the gastrointestinal tract of the animal an effective amount of an altered wheat starch in the form of or derived from the grain of a wheat plant. The proportion of amylose in the starch of the grain is at least 30% and/or the grain comprises a reduced level of SBEIIa enzyme activity and/or protein relative to wild-type grain.

Method and means for improving bowel health

Wheat with altered branching enzyme activity and starch and starch containing products derived therefrom

A method and composition for improving one or more indicators of bowel health or metabolic health in a mammalian animal. This comprises the delivering to the gastrointestinal tract of the animal an effective amount of an altered wheat starch in the form of or derived from the grain of a wheat plant. The proportion of amylose in the starch of the grain is at least 30%.

Barley having a reduced level of SBEIIa activity produces grain having a high relative amylose content. The barley might additionally have reduced levels of SBEIIb activity. The barley grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway.

Growing community of inventors

Palmerston, Australia

Ahmed Regina