The Ohio State University

Kraft Foods Holdings, Inc.

Ahmed E Yousef

David Kasler

Mohammad Shavezipur

Luis A Rodriguez-Romo

Chunhua Yuan

Liwen Zhang

Tarik A Yousef

Yaoqi Guo

En Huang

Osama O Ibrahim

Hyun-Jung Chung

Emily Campbell

An apparatus produces a target metabolite in a bioreaction. The apparatus has a reactor vessel that contains a bulk liquor in which the bioreaction is conducted, with a headspace provided above the bulk liquor. In some instances, the target metabolite is favorably partitioned into a foam formed at an interface of the bulk liquor and the headspace. A foam collector is arranged to receive this foam from the bioreactor and break it into a condensed foam liquid, from which the target metabolite may be isolated and purified. In one exemplary application, paenibacillin is produced by fermentation of a medium byOSY-DF, and is preferentially concentrated in the foam. In another exemplary application, fermentation of a medium byGF610 produces amyloliquecidin GF610 is which is preferentially concentrated in the foam.

Automated bioreactor with foam collector

A thermal simulator simulates the thermal behavior of items such as eggs for which the actual internal temperature profile is difficult to measure. A yolk body simulates the egg yolk, an albumen body surrounds the yolk body and simulates the egg albumen, and a shell layer surrounds the albumen body to simulate the shell. The thermal properties of the materials forming the egg body, albumen body and shell layer are tuned to match the thermal properties of the egg yolk, egg albumen and egg shell. Thermometric devices are positioned within the egg body and egg albumen along with communication devices which process signals from the thermometric devices indicative of temperature and communicate these signals to a computer for further processing and display.

Thermal simulator

Coatings for shell eggs improve the strength and shatter resistance of processed eggs. The coatings include food grade natural resins such as shellac, mixtures of paraffin and beeswax, and food grade polymer emulsions such as polyvinyl acetate.

Coated shell eggs and method of making same

Provided herein is a biologically pure culture of, identified as OSY-SE, as well as an antimicrobial agent isolated and/or purified from the culture having any one of SEQ ID NOs:1-3 and 64-66. The disclosure also provides compositions and articles of manufacture comprising an antimicrobial agent and/or the bacterial cell of, identified as OSY-SE. Further provided are methods of use, including methods of affecting microbial activity, methods of inhibiting growth and/or proliferation of a microbe, methods of treating a condition or disease associated with the presence of a microbe, and methods of treating a microbial infection in a subject comprising contacting a microbial cell with at least one active agent of SEQ ID NOs:1-3 and 64-66 and/or the bacterial cell, identified as OSY-SE. The disclosure also provides the biosynthetic machinery (e.g., utilizing a NRPS mechanism) including isolated proteins, isolated polynucleotides, vectors, and host cells for production of the antimicrobials described herein.

Antimicrobial agent, bacterial strain, biosynthesis, and methods of use

Methods for reducing external and internal contamination of shell eggs by utilizing gaseous ozone applied under mild pressure, alone or in combination with UV radiation for external contaminants and gaseous ozone under mild pressure, a mix of carbon dioxide and ozone, heat, vacuum, or combinations thereof for internal contaminants.

Process for ozone-based decontamination of shell eggs

The present invention is directed bacterial isolates that produce proteinacious antimicrobial agents effective against a variety of food borne pathogens. The invention includes the bacteria, bacteriocin preparations derived from the bacteria and methods by which the preparations may be used.

Intracellular proteinacious antimicrobial agents from lactic acid bacteria derived from fermented food samples

Methods for reducing external and internal contamination of shell eggs by utilizing gaseous ozone applied under mild pressure, alone or in combination with UV-light for external contaminants and gaseous ozone under mild pressure, a mix of carbon dioxide and gaseous ozone, heat, vacuum, or combinations thereof for internal contaminants.

Growing community of inventors

Columbus, OH, United States of America

Ahmed E Yousef