Nanobiotix

Centre National De La Recherche Scientifique

Universitë De Bordeaux

Agnés Pottier

Laurent Levy

Marie-Edith Meyre

Matthieu Germain

Audrey Darmon

Cyril Lorenzato

Chrit Moonen

Pierre Smirnov

The present invention relates to a pharmaceutical composition comprising the combination of (i) a biocompatible nanoparticle and of (ii) a pharmaceutical compound of interest, to be administered to a subject in need of such a compound of interest, wherein the nanoparticle potentiates the compound of interest efficiency. The longest dimension of the biocompatible nanoparticle is typically between about 4 and about 500 nm, and its absolute surface charge value is of at least 10 mV (|10 mV|). The invention also relates to such a composition for use for administering the compound of interest to a subject in need thereof, wherein the nanoparticle and the compound of interest are to be administered to said subject between more than 5 minutes and about 72 hours from each other.

Pharmaceutical composition, preparation and uses thereof

The invention pertains to a method of monitoring the membrane permeabilization of a liposome and the incidental release of a compound of interest. The method utilizes liposomes comprising a thermosensitive lipidic membrane encapsulating the product of interest and superparamagnetic nanoparticles having the electrostatic surface charge below &#x2212;20 mV or above +20 mV when measured in an aqueous medium at physiological pH. In one embodiment, the method comprises the steps of: a) measuring relaxation time (T2*); b) heating the liposome at Tm or above Tm; c) measuring T2* after step b); d) obtaining the transverse relaxivity (r) values from the T2* values obtained from step a) and step c); and e) determining the ratio of rbefore and after the heating step b). A ratio above 1.5 indicates the liposome membrane permeabilization and the incidental release of the product of interest.

Method of monitoring the release from liposomes of a product of interest using superparamagnetic nanoparticles

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Paris, France

Agnés Pottier