Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Institut National De La Sante Et De La Recherche Medicale

Centre Hospitalier Universitaire De Grenoble

Universite Jospeh Fourier

Université Joseph Fourier

Affif Zaccaria

Patrice Caillat

François Berger

Raymond Campagnolo

Marie-Line Cosnier

The invention relates to a device for transiently contacting at least one unit for capturing biological targets with a body fluid containing them, a method for recovering the captured targets for analysis, and a system for contacting and recovering a capture substrate included in said unit. The invention relates to samples obtained in particular in vivo from body fluids of the human body, e.g. circulating body fluids, said fluids possibly containing, as targets, proteins, oligonucleotides such as RNA or DNA, antibodies, enzymes or cells. A contacting device () according to the invention comprises: &#x2014;a sampling tip () having a contacting end intended to be introduced into a medium containing said fluid, and&#x2014;the or each capturing unit which is bound to said end and which comprises a capture substrate () defining a target capturing surface covered with at least one biocompatible and porous crosslinked polymer layer () designed to retain the or each capture substrate and only to allow biological particles including targets smaller than a limiting size to pass through, in such a way that the or each capture substrate is recoverable after said contacting by dissolution of the or each layer.

Device for transiently contacting at least one unit for capturing biological targets with a fluid containing them, method for recovering the captured targets and system for contacting and recovery

Growing community of inventors

Le Pont de Claix, France

Affif Zaccaria