Dsm IP Assets B.v.

Dsm N.v.

Adrianus W H Vollebregt

Masako Shinjoh

Bertus Pieter Koekman

Nigel John Mouncey

Dick Schipper

Connie Lee

Corina Hauk

Anne F Mayer

Anja Meury

Christine Toepfer

Markus G Goese

Marie-Gabrielle Beuzelin-Ollivier

Manuela Dalluege

Marina Van Gelder

Bastien Chevreux

Bart Willem Swinkels

Janna Gardina Bakhuis

Bastian Chevreux

Gerardus C M Selten

Roelof A L Bovenberg

The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

Fermentative Vitamin C production

Fermentative vitamin C production

The present invention discloses novel amdS genes from fungi previously not known to contain an amdS gene, such as and . The novel amdS genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amdS genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.

Polynucleotides encoding Aspergillus Niger and Penicillium Chrysogenum acetamidases and methods of use as selectable markers

Growing community of inventors

Naaldwijk, Netherlands

Adrianus W H Vollebregt