Pacific Biosciences of California, Inc.

Siemens Healthcare Diagnostics Gmbh

Singular Bio, Inc.

Invitae Corporation

Adrian Nielsen Fehr

Stephen Wilson Turner

Nathaniel McCaffrey

Patrick James Collins

Hywel Bowden Jones

Jill Lyndon Herschleb

Jeremy Gray

Thomas Battersby

Minxue Zheng

Jonas Korlach

Robin A Emig

Jeffrey Wegener

Benjamin A Flusberg

Arunashree Bhamidipati

Robert Sebra

Keith P Bjornson

Kevin Travers

Thang Pham

Yu-Chih Tsai

Nathaniel Joseph Mccaffrey

Mathieu Foquet

Fred C Christians

John S Eid

Jon M Sorenson

Lei Sun

Stephen P Dudek

Hans Callebaut

Eric Olivares

Bidhan P Chaudhuri

Steven Lin

Ravi Dalal

Aj Chmura

Albert James Chmura

Pham Thang

An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.

Illumination of integrated analytical systems

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

Optics collection and detection system and method

The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes. The invention also relates to manufacturing and using molecular arrays and analytical approaches based on single molecule detection techniques.

Arrays for single molecule detection and use thereof

The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes.

Assays for single molecule detection and use thereof

The present disclosure relates to normalization of biological samples, particularly samples comprising nucleic acids to be sequenced. The normalization protocols described herein may be utilized across multiple samples to cap total stoichiometric input and minimize variations in transcript abundance on a per-sample basis in a multiplexed fashion to dramatically increase the accuracy, capacity and efficiency of nucleic acid sequencing.

Method of normalizing biological samples

The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. The invention includes devices having sample and reference pores connecting sample, measurement and reference chambers, wherein potential measurements in each chamber is used to provide an accurate determination of current through a sample nanopore, improving nanopore sequencing.

Nanopore sequencing using ratiometric impedance

The method includes obtaining a sample including a target nucleic acid, contacting the sample with a quantification primer, adding a quantity of blocking primer to the sample, and repeating the contacting and adding steps until the total quantity of blocking primer present in the sample meets or exceeds the amount of target present in the sample. Each repetition utilizes a quantification primer having a different unique identifying feature assigned to sequentially increasing quantification levels, and the quantity of blocking primer added at each repetition establishes numerical spacing between the quantification levels. The method further includes identifying the unique identifying feature present in any bound quantification primers that remain in the sample, where the presence of a unique identifying feature assigned to a particular quantification level indicates the approximate amount of the target present in the sample.

Method of quantitating the amount of a target nucleic acid in a sample

Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.

Apparatus for loading molecules onto substrates

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San Francisco, CA, United States of America

Adrian Nielsen Fehr