Medical Research Council

Adrian Huber

Gregory Paul Winter

Dario Neri

Dan S Tawfik

Francesca Viti

Salvatore Demartis

A method for isolating the DNA encoding an enzyme is presented. The DNA is contained within a support to which the enzyme is linked, wherein the enzyme is further linked to a substrate. When the enzyme reacts with the substrate, the product remains linked to the enzyme. The product linked to the enzyme which is linked to the support containing the DNA encoding the enzyme is isolated, thereby isolating the DNA encoding the enzyme. In this manner an expression library of enzymes produced by mutagenesis can be screened for mutated active enzyme, and thereby obtain the mutated DNA encoding the mutated active enzyme.

Method for isolating the DNA encoding an enzyme

The present invention relates to a method of isolating an enzyme having a desired chemical or biological activity, the method comprising linking the enzyme to a substrate for the enzyme, reacting the enzyme and substrate such that a product is produced which remains linked to the enzyme and isolating the enzyme by selectively isolating the product linked to the enzyme.

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Zurich, Switzerland

Adrian Huber