Ventana Medical Systems, Inc.

Genentech, Inc.

National Cancer Center

Adrian E Murillo

Jerome W Kosmeder, Ii

Christopher Bieniarz

Atsushi Ochiai

Julia Ashworth-Sharpe

Chol Steven Yun

Michael Patrick Farrell

Brian Daniel Kelly

Donald D Johnson

Takeshi Kuwata

Zhanna Zhilina

Nathan Walter Polaske

Yuri Belosludtsev

Traci D DeGeer

Wendy J French

Eric J May

Hiro Nitta

Junshan Hao

Pascal Bamford

Donald G Munroe

Akio Kaito

Jerome W Kosmeder

Amy A Lo

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

Antibody-nanoparticle conjugates and methods for making and using such conjugates

Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens

Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.

Application of quantum dots for nuclear staining

This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formula where R, R, R, and Rare independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; Rand/or Rcan be bound to Rto form a fused, aromatic ring system; Ris selected from a heteroatom-containing moiety; A is selected from, a carbon atom, a heteroatom, other than sulfur, and any combination thereof; n is 1-5, an enzyme, a specific binding moiety and a detectable moiety.

Enhanced deposition of chromogens utilizing pyrimidine analogs

Enhanced deposition of chromogens

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Tucson, AZ, United States of America

Adrian E Murillo