Allosource

Other

Adrian C Samaniego

Matthew Southard

Reginald L Stilwell

Kenneth Blood

Brent Lee Atkinson

Jan Zajdowicz

Matthew Peterson

Timothy Bennett

Wendy Desiree Franklin

Bruce Mock

Cathleen Lucine Badillo

Ross M Wilkins

Aidan Griffin

Joslynn Shamis

Luke Shubin

Richard Dempsey

Lauren Blume

William Maslanik

Peter J Stevens

Carolyn Barrett Rorick

Yurid Gourgel

Marina Bull

Bilal Karaze

Sarah Song Yi Han

Arthur Joslyn

Jesse Compton

Cole Watkins

Teresa C Romansky

Cameron Mattson

Megan Maccagnan

Matt Southard

Jessica Hodgkiss

Matthew James Southard

Marina Katelyn Bull

Kathryn Hanzlicek

Systems and methods for the automated demineralization of bone and decellularization of soft tissue include a mixing assembly communicatively coupled with a control and reporting system. The mixing assembly includes a housing that supports a cannister sub-assembly forming a mixing chamber having a reagent inlet, a reagent outlet, and an internal fluid agitator. A motor is operably coupled with the agitator and configured to spin the agitator in first and second directions relative to the cannister, thereby agitating fluid contained within the mixing chamber. At least one pump is configured to progressively inject measured quantities of a plurality of reagents into the mixing chamber. The associated control and reporting system includes a user terminal that displays a graphical user interface enabling a user to initiate an automated demineralization or decellularization procedure in which the mixing assembly demineralizes tissue placed within the mixing chamber. Other embodiments are also disclosed.

Automated system and methods for tissue demineralization

Periosteum harvesting and processing systems and methods enable rapid, efficient, and repeatable harvesting of periosteum tissue from human long bones and processing of the tissue into individual fibers for use in strengthening surgical allograft products. One harvesting and processing method involves providing a human long bone, securing the long bone between two rotating live centers, scraping, using one of a plurality of harvesting tools selected from a harvesting and processing kit, periosteum tissue from the long bone, executing a first wash cycle comprising a hydrogen peroxide wash, executing a second wash cycle comprising an isopropyl alcohol wash, executing a third wash cycle comprising a phosphate buffered saline wash, compressing the periosteum tissue to remove excess fluid, cryofracturing the tissue until a desired fiber size is achieved, and separating the periosteum tissue into individual periosteum fibers having the desired fiber size. Other embodiments are also disclosed.

System and methods for harvesting and processing periosteum fibers

Embodiments of this technology may include an apparatus for drying tissue. The apparatus may include human donor tissue placed in contact with and between at least two layers of backing material. This tissue and backing layer may then be restrained by two plates. This tissue may be amniotic tissue. The backing layer may include woven or nonwoven material. This backing layer may be wetted with a saline solution. At least one of the plates of the tissue drying apparatus may be perforated. The tissue assembly along with the plates may then be placed inside a chamber configured to receive the plates and tissue assembly. The chamber may be configured so that gas can be forced into the chamber with the gas flow going around the plates and the tissue assembly.

Systems for drying sheets of donor-provided human tissue

Embodiments of the present technology may permit for native, intact, natural, human-derived, or allograft-derived fibers to be used as threads for suturing. Embodiments may include a thread for suturing. The thread may include a first portion, which includes a fascia fiber. The first portion may have a first end that includes the fascia fiber. The thread may also include a second portion including a non-human-derived fiber. The second portion may have a first end that includes the non-human-derived fiber. The first end of the second portion may be attached to the first end of the first portion. Embodiments may include a method of forming a thread. The method may include attaching a first end of a fascia fiber to a first end of a first non-human-derived fiber. The method may also include attaching a second end of the fascia fiber to a first end of a second non-human-derived fiber.

Fascia fibrous compositions and methods for their use and manufacture

Embodiments of the present technology include a method of making a bone composite graft for administration to a patient. The method may include combining a human cadaveric bone material with a plurality of polymethyl methacrylate binder particles and spincasting the combined human cadaveric bone material and polymethyl methacrylate binder particles to produce the bone composite graft. The method may also include ablating the bone composite graft to increase the surface area of bone material exposed. The human cadaveric bone material may be immobilized in the plurality of polymethyl methacrylate binder particles. The human cadaveric bone material may be present in an amount that is 50 weight percent of the bone composite graft, or less. Additionally, the bone composite graft may have a yield strength that is at least 13,000 N/cm2 and no greater than 15,000 N/cm2.

Composite bone constructs and methods

Composite grafts including a biocompatible, synthetic scaffold; and a biological tissue component obtained or derived from a deceased donor tissue, wherein the biological tissue component is embedded in the biocompatible, synthetic scaffold, are provided as systems relating thereto. Methods of manufacture and methods of treatment using such grafts are also provided.

Composite grafts, systems, and methods

There is disclosed a customizable system and methods of use for manufacturing and testing a variety of pre-sutured allograft tendon constructs sutured according to a variety of stitching patterns and featuring a variety of tissue lengths and types. One embodiment includes a triple-channel base having first, second, and third longitudinal channels extending between first and second ends of the base, as well as a plurality of tendon-manipulation accessories. Each of the tendon-manipulation accessories includes a locking-base assembly for selectively securing the accessory to the base such that the multiple tendon-manipulation accessories may be secured to the triple-channel base in a variety of custom arrangements suitable for preparing the variety of pre-sutured constructs and/or for pre-tensioning or testing the variety of the pre-sutured constructs. Other embodiments are also disclosed.

System and methods of use for preparing and testing pre-sutured tendon constructs

Flowable matrix compositions and methods of their use and manufacture are provided. Exemplary compositions may include a flowable, syringeable, putty-like form of acellular human dermal matrix. In some cases, compositions may include a moldable acellular collagen extracellular matrix. In use, the matrix compositions can be used to fill or treat skin voids, channel wounds, and other soft tissue deficiencies.

Flowable matrix compositions and methods

Embodiments of the technology may involve a method of processing the human donor tissue for administration to a recipient. This method may include the step of contacting the human donor tissue with a backing layer, where the human donor tissue and the backing layer contain a saline solution. This saline solution may include a solvent and a disassociated salt. The method may further include evaporating a portion of the solvent from a surface of the backing layer. The evaporation of the solvent may move a portion of the disassociated salt from the donor tissue to the backing layer. This process may then result in a tissue that is mostly dry and free of salt crystals.

Methods of drying sheets of donor-provided human birth tissue

Embodiments of the present technology include a graft for administration at a treatment site of a patient. The graft may include a human cadaveric bone material bonded together with a polymeric binder. The human cadaveric bone material may include demineralized bone particles. The demineralized bone particles may have an average diameter less than 1.1 mm, less than 750 &#x3bc;m, less than 500 &#x3bc;m, or less than 250 &#x3bc;m. The human cadaveric bone material may include non-demineralized bone, cancellous bone, and/or cortical bone in embodiments. In some embodiments, bone from animals other than humans may be used, and the patient may be an animal other than a human.

Embodiments of the present invention encompass anti-adhesion wound dressings including patches made from amnion tissue obtained from human birth tissue. Exemplary amnion patches can be fabricated by folding a section of amnion over on itself with the epithelial layer on the outside of the folded patch and the fibroblast layer on the inside of the folded patch. Optionally, individual amnion tissue pieces can be sandwiched together to provide a multi-layer patch. Sufficient pressure is applied to the layered amnion to cause adherence between opposing faces of the fibroblast layers. The pressed fibroblast layers provide mechanical strength to hold the amnion patch together with the epithelial layers on the outsides of the amnion patch.

Multi-layer tissue systems and methods

There is disclosed an apparatus and method of manufacture relating to a pre-sutured, high-strength allograft tendon construct. One embodiment of the allograft tendon construct includes first and second tendon lengths that are positioned longitudinally in parallel to one another. A whip stitched pattern secures the first and second tendon lengths at a free end, forming a stitched end portion that abuts an unstitched middle portion. The whip stitched pattern includes a plurality of sutures that originate adjacent to the free end and progress or advance inward toward the unstitched middle portion. A multiple knot bundle is applied immediately prior to a final suture, such that the final suture loops about the multiple knot bundle and locks the multiple knot bundle in place beneath the final suture. Other embodiments are also disclosed.

High-strength allograft tendon construct

Growing community of inventors

Parker, CO, United States of America

Adrian C Samaniego