California Institute of Technology

Chromacode, Inc.

Sanofi

The Boeing Company

Aditya Rajagopal

Axel Scherer

Emil Paskalev Kartalov

Thomas Anthony Tombrello, Jr

Alaina Ann Brinley Rajagopal

Andrew Peter Homyk

Chieh-feng Chang

Sameer Walavalkar

Erika F Garcia

Samson Chen

Mark D Goldberg

Seheon Kim

Xiomara Linnette Madero

Michael David Henry

Stefan Petry

Oliver Plettenburg

Christopher I Walker

Yaser Said Abu-Mostafa

Charles L Tschirhart

Mladen Barbic

Akram Sarwat Sadek

Christopher Macdonald

Dominic Yurk

Leora Peltz

Lucien A E Jacky

Gregory Gosch

Raymond Jimenez

Michael Woods

Paul Flook

Karen L Menge

Lucien AE Jacky

Some implementations of the disclosure describe a blood pressure measurement apparatus and method that enable continuous, non-invasive blood pressure measurement using sound and ultrasound transducers. In one implementation, a blood pressure measurement device includes: a first transducer configured to emit multiple soundwaves having multiple frequencies, the soundwaves configured to cause a blood vessel of a subject to vibrate; a second transducer configured to capture one or more ultrasound images of the blood vessel; and a processing device configured to: determine, based on the one or more ultrasound images, a wall thickness, a radius, and a resonant frequency of the blood vessel; and calculate, based on the wall thickness, the radius, and the resonant frequency, a blood pressure of the subject.

Blood pressure measurement apparatus and methods of use thereof

The present disclosure provides methods and compositions for multiplex quantitation. In some aspects, methods and compositions are provided for quantitation of nucleic acid targets from a biological sample. The disclosed methods may be useful in identifying or detecting genetic abnormalities from a subject.

Methods and systems for multiplex analysis

A measurement device includes: a processing device and multiple sensors that capture tomographic information of a physiological structure. The sensors include a first sensor including a first transducer having a first frequency response with a first resonant frequency, and a second sensor including a second transducer having a second frequency response with a second resonant frequency different from the first resonant frequency. The first frequency response partially overlaps with the second frequency response. The second transducer transmits a signal that is reflected by the physiological structure to create a reflected signal, the first transducer generates a first received signal from the reflected signal, the second transducer generates a second received signal from the reflected signal, and the processing device normalizes the first received signal with the second received signal or the second received signal with the first received signal.

Measurement device

The present disclosure provides methods and compositions for the multiplexed detection of multiple analytes from a sample. Analytes may be nucleic acid analytes. Detection of analytes may comprise contacting one or more sample subsets with hybridization probes, thereby generating one or more cumulative signal measurements capable of detecting the presence of absence of a plurality of analytes.

Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

Signal encoding and decoding in multiplexed biochemical assays

An ultrasound measurement device includes: a processing device and multiple ultrasound sensors that capture tomographic information of a physiological structure. The ultrasound sensors include a first ultrasound sensor including a first transducer having a first frequency response with a first resonant frequency, and a second ultrasound sensor including a second transducer having a second frequency response with a second resonant frequency different from the first resonant frequency. The first frequency response partially overlaps with the second frequency response. The second transducer transmits an ultrasound signal that is reflected by the physiological structure to create a reflected ultrasound signal, the first transducer generates a first received signal from the reflected ultrasound signal, the second transducer generates a second received signal from the reflected ultrasound signal, and the processing device normalizes the first received signal with the second received signal or the second received signal with the first received signal.

Ultrasound measurement device

An enhanced stethoscope device and method for operating the enhanced stethoscope are provided. The enhanced stethoscope device generally operates by providing stethoscope sensors, ultrasonic sensors, and other sensors to obtain a series of measurements about a subject. The series of measurements may be correlated, such as by machine learning, to extract clinically relevant information. Also described are systems and methods for ultrasonic beamsteering by interference of an audio signal with an ultrasonic signal.

Enhanced stethoscope device and method for blood pressure measurement via plurality of ultrasound transducers and optical sensors to determine time series blood values using autoencoders

A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

Chromophore-based detection of genetic variations in analytes

Some implementations of the disclosure are directed to an ultrasound measurement device including: multiple ultrasound sensors to capture tomographical information of a physiological structure, each ultrasound sensor comprising a transducer having a respective resonant frequency, where each transducer has a frequency response that partially overlaps with a frequency response of another transducer; and a processing device to control and process measurements made by the ultrasound sensors. The device may be incorporated in an adhesive substrate configured to be adhered to a patient's skin in alignment with an artery of the patient. The processing device may use the multiple ultrasound sensors to compute the mean arterial pressure through the artery by performing operations of: measuring a circumference of the artery using the multiple ultrasound sensors; measuring a blood flow velocity using the same ultrasound sensors; and computing the mean arterial pressure using the measured arterial circumference and blood flow velocity.

Wireless ultrasound monitoring device

The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.

Nucleic acid reactions and related methods and compositions

Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

Chromophore-based medical system for detecting genetic variations in analytes

FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the F&#xf6;rster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

Growing community of inventors

Irvine, CA, United States of America

Aditya Rajagopal