The University of Texas System

Leland Stanford Junior University

The General Hospital Corporation

Adela Ben-Yakar

Sertan Kutal Gökçe

Navid Ghorashian

William Neil Everett

Sudip Mondal

Evan Hegarty

Sam Xun Guo

Christopher Martin

Christopher L Hoy

Frederic Bourgeois

R Rox Anderson

Olav Solgaard

William A Farinelli

Steven M Zeitels

Xun Guo

Ki Hyun Kim

Tianqi Li

Chris Martin

Peisen Zhao

James B Kobler

Daniel Eversole

Sertan Kutal Gokce

Systems and methods for line excitation array detection (LEAD) microscopy. The systems and methods include an excitation beam from an optical beam source and a subject of interest. Light is scanned across the subject of interest and optical signals are detected using a parallel optical detection means. A number of mechanical, acoustic and/or optical components such as scanning mirrors, DMDs, OADs, electric motors may be used separately or in conjunction to aid in the scanning of the excitation beam across the subject of interest.

Line excitation array detection microscopy

A microfluidic device capable of trapping contents in a manner suitable for high-throughput imaging is described herein. The microfluidic device may include one or more trapping devices, with each trapping device having a plurality of trapping channels. The trapping channels may be configured to receive contents via an inlet channel that connects a sample reservoir to the trapping channels via fluid communication. The trapping channels are shaped such that contents within the trapping channels are positioned for optimal imaging purposes. The trapping channels are also connect to at least one exit channel via fluid communication. The fluid, and contents within the fluid, may be controlled via hydraulic pressure.

High-throughput imaging platform

A laser scanning system for capturing an image of a specimen is described herein. The laser scanning system includes a light source configured to emit a light beam for illuminating the specimen, a scanning unit including a plurality of reflectors for scanning the light beam along first and second axes, and a data acquisition unit configured to control acquisition of the image. The laser scanning system can include a control circuit configured to receive a reference clock signal for the first reflector and generate a synchronization clock signal based on the reference clock signal. The laser scanning system can include a synchronization controller configured to control the scanning unit and the data acquisition unit. The synchronization controller can be configured to receive the synchronization clock signal, receive a plurality of imaging parameters, and generate a plurality of control signals based on the synchronization clock signal and the imaging parameters.

High-speed laser scanning microscopy platform for high-throughput automated 3D imaging and functional volumetric imaging

Microfluidic devices for the rapid and automated processing of sample populations are provided. Described are multiplexer microfluidic devices configured to serially deliver a plurality of distinct sample populations to a sample processing element rapidly and automatically, without cross-contaminating the distinct sample populations. Also provided are microfluidic sample processing elements that can be used to rapidly and automatically manipulate and/or interrogate members of a sample population. The microfluidic devices can be used to improve the throughput and quality of experiments involving model organisms, such as

Microfluidic devices for the rapid and automated processing of sample populations

Provided herein are devices, systems and methods for treating a vocal fold pathology by forming a substantially planar void below the epithelium of the vocal fold using optical energy. Also provided are devices, systems, and methods for combined imaging and treating of a vocal fold pathology.

Systems, devices and methods for imaging and surgery

Provided herein are devices, systems and methods for imaging of biological tissue. Also provided are devices, systems and methods for surgical manipulation of biological tissue. Further provided are devices, systems and methods for combined imaging and surgical manipulation of biological tissue.

A novel femtosecond laser nano-ablation technique called Plasmonic Laser Nano-Ablation (PLN). The technique takes advantage of surface-enhanced plasmonic scattering of ultrashort laser pulses by nanoparticles to vaporize sub-cellular structures in attoliter volumes. The use of nanoparticles may overcome problems associated with current FLMS techniques and does not rely on heating for nanodisruption. In PLN, the particle acts as a 'nano-lens,' restricting laser light to the near-field of the particle, and only photodisrupting structures that are nanometers away. This eliminates the need for a tightly focused beam, while still achieving nanoscale ablation resolution. Moreover, the enhanced scattering around the particles reduces the amount of required laser fluence. A method is provided comprising positioning a nanoparticle in proximity to a surface of a material; irradiating the nanoparticle with a laser tuned close to the nanoparticle's plasmonic frequency; and allowing a near-field effect from the irradiated nanoparticle to photodamage the material.

Plasmonic laser nanoablation methods

Growing community of inventors

Austin, TX, United States of America

Adela Ben-Yakar