Carnegie Mellon University

Harvard College

University of Florida Research Foundation, Incorporated

University of Pittsburgh

President and Fellows of Havard College

Adam Walter Feinberg

Kevin Kit Parker

Thomas Hinton

Josue A Goss

Anna Grosberg

Patrick W Alford

Anthony B Brennan

Thomas Gregory Estes

James Frederick Schumacher

Ronald Howard Baney

Mark Daniel Brigham

Leslie H Wilson

Andrew Lee

Andrew Hudson

George M Whitesides

Sergey S Shevkoplyas

Johan Ulrik Lind

Po-Ling Kuo

Michelle L Carman

Ashutosh Agarwal

Megan Laura McCain

Rebecca Duffy

Rachelle Simko

Jacqueline Wittmer

Alexander Feigel

Michelle Carman Turnage

James L Funderburgh

Chin-Lin Guo

Maria Stang

A method for micro-tissue encapsulation of cells includes coating a tissue scaffold stamp with an extracellular matrix compound; depositing the tissue scaffold stamp onto a thermoresponsive substrate; seeding the tissue scaffold stamp with a cell culture; incubating the cell culture on the tissue scaffold stamp at a temperature that is specified, wherein the cell culture forms a cell patch that is attached to the extracellular matrix compound; removing the thermoresponsive substrate by lowering the temperature; removing the tissue scaffold stamp from the cell patch to form a micro-tissue structure by dissolving the tissue scaffold stamp in a solvent; folding the micro-tissue structure by suspending the micro-tissue in the solvent to enable the cell patch to fold the micro-tissue structure; collecting the folded micro-tissue structure from the solvent; and administering the folded micro-tissue structure to an organism.

Extracellular matrix scaffolds

In one aspect, a method includes providing support material within which the structure is fabricated, depositing, into the support material, structure material to form the fabricated structure, and removing the support material to release the fabricated structure from the support material. The provided support material is stationary at an applied stress level below a threshold stress level and flows at an applied stress level at or above the threshold stress level during fabrication of the structure. The provided support material is configured to mechanically support at least a portion of the structure and to prevent deformation of the structure during the fabrication of the structure. The deposited structure material is suspended in the support material at a location where the structure material is deposited. The structure material comprises a fluid that transitions to a solid or semi-solid state after deposition of the structure material.

Additive manufacturing of embedded materials

A method and system for additive manufacturing are provided herein. The method comprises depositing a structure material, by a nozzle (), into a support material () by applying to the structure material such that the structure material flows through the nozzle. The structure material comprises a polymer and a rheological modifier. Depositing of the structure material is repeated as necessary to create an object (). The support material is at least partially removed from object. In various examples, the method comprises varying a print parameter from a first portion of the object to a second portion of the object, moving the nozzle away from a previously deposited layer of the object when repositioning for deposition of a subsequent layer of the object, or a combination thereof.

Modification of rheology and machine pathing for improved 3D printing of soft materials

This document describes systems and methods for integrated mechanical loading of tissue. The system includes a three-dimensional tissue comprising organic material. The system includes a strip of bendable material. The strip includes a first region proximate to a first end of the strip coupled to the tissue. The strip includes a second region near a second end of the strip for coupled to the tissue, the second end being opposite the first end, wherein the tissue exerts a force on the strip to bend the strip, the force caused by contraction of the tissue, and wherein the strip exerts a stress on the tissue.

Three-dimensional microtissues with integrated mechanical loading

This document describes a process of producing gel microparticles, which are consistent in size and morphology. Through the process of coacervation, large volumes of gel microparticle slurry can be produced by scaling up reactor vessel size. Particles can be repeatedly dehydrated and rehydrated in accordance to their environment, allowing for the storage of particles in a non-solvent such as ethanol. Gel slurries exhibit a Bingham plastic behavior in which the slurry behaves as a solid at shear stresses that are below a critical value. Upon reaching the critical shear stress, the slurry undergoes a rapid decrease in viscosity and behaves as a liquid. The rheological behavior of these slurries can be adjusted by changing the compaction processes such as centrifugation force to alter the yield-stress. The narrower distribution and reduced size of these particles allows for an increase in FRESH printing fidelity.

Additive manufacturing support material

This document describes systems and method of embedded printing for additive manufacturing. A print material is printed into a support material. The print material and the support material each have a fluid phase and a solid phase. The print material transitions from the fluid phase to the solid phase based on a fluid-fluid interaction with the support material. One or more parameters of the support material can be adjusted to cause a diffusion rate of the print material into the support material during the fluid-fluid interaction to be less than a threshold value. Multiple print materials can be printed into the support material simultaneously.

Additive manufacturing based on fluid-fluid interface

The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

Devices comprising muscle thin films and uses thereof in high throughput assays for determining contractile function

Muscle chips and methods of use thereof

A highly tunable bioscaffold is provided, as well as a method of manufacture of the bioscaffold and methods of use of the bioscaffold, for example for drug testing, cell propagation and for optimizing growth of a cell type, for example corneal endothelial cells.

Polysiloxane substrates with highly-tunable elastic modulus

The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for these assays.

Growing community of inventors

Pittsburgh, PA, United States of America

Adam Walter Feinberg