Bio-rad Laboratories, Inc.

Affymetrix, Inc.

Adam McCoy

Yan Wang

Xiao-Song Gong

Man Cheng

Heather Koshinsky

Philip H Dickinson

John D Curry

Daniel Fitzpatrick

Anthony C Schweitzer

Robert O'Callahan

Benjamin J Hindson

Serge Saxonov

Simant Dube

Svilen Tzonev

Niels Klitgord

Frank Hsiung

Disclosed are compositions, methods and kits for determining the presence, absence, amount, copy number, or other characteristics of one or more polynucleotide sequences in two or more samples and use thereof in genotyping, evaluation of copy number variation, expression analysis, determination of splice variants and fusion genes, and other genetic analyses.

Nucleic acid analysis by joining barcoded polynucleotide probes

Methods of detecting and amplifying short RNAs are provided.

RNA amplification methods

System, including methods, apparatus, and compositions, for performing a digital assay with associated targets. In some embodiments, the assay of associated targets in partitions may lower the limit of detection (LOD) or otherwise increase assay sensitivity, accuracy, and/or specificity. The targets may represent spaced or overlapping regions of the same template and/or each may represent a region from a different complementary strand of the same template. In some embodiments, the associated targets may be provided by a type of biological particle, and the target content of partitions may be used to identify and quantify the type of biological particle in a sample.

Digital assays with associated targets

The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Affinity-based partition assay for detection of target molecules

Methods, compositions, and kits are provided for quantifying RNA molecules in a partitioned sample via multiple reverse transcription reactions.

Repetitive reverse transcription partition assay

System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

Multiplexed digital assays with combinatorial use of signals

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Davis, CA, United States of America

Adam McCoy