10X Genomics, Inc.

Bio-Rad Laboratories, Inc.

Han, Chunxiao

Hiddessen, Amy, L.

Hindson, Benjamin, J.

Chia, Erin, R.

Lowe, Adam

Adam Lowe

Christopher Hindson

Shalini Gohil

Joshua Delaney

Benjamin J Hindson

Andrew D Price

Amy L Hiddessen

Chunxiao Han

Erin Rae Chia

Joseph Francis Shuga

Meiliana Tjandra

Albert Dale Kim

Geoff McDermott

Jill Herschleb

Yi Luo

Yaqi Wang

Ben Hindson

Wyatt James Mcdonnell

Michael John Terry Stubbington

Chris Hindson

Erin Chia

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

Methods for generating barcoded nucleic acid molecules using fixed cells

Compositions and methods for using fixed biological samples

The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

Compositions, methods, and systems for bead formation using improved polymers

Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Compositions, methods and systems for polymerase chain reaction assays

System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

Silicone surfactants for emulsion assays

The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays. The present disclosure provides methods, devices, systems and compositions for detecting nucleic acids in ddPCR assays using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Growing community of inventors

Mountain House, CA, United States of America

Adam Lowe