University of Minnesota

Iowa State University Research Foundation, Inc.

Cornell Research Foundation Inc.

Cornell University

Basf Agricultural Solutions Seed US LLC

Adam J Bogdanove

Daniel F Voytas

Feng Zhang

Steven V Beer

Zhong-Min Wei

Jihyun Francis Kim

Alan Collmer

Manuel Dubald

David W Bauer

Clarice Lauer Schmidt

Erin Doyle

Li Wang

Tomas Cermak

Michelle Christian

Jong Hyun Ham

Alain Sailland

Fabio Rinaldi

Yueh-Jiang Hwang

Apurva Bhargava

The present disclosure relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in a plant cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that comprises a DNA binding domain comprising one or more Transcription Activator-Like (TAL) effector monomers specifically ordered to target the genomic locus of interest to improve tolerance of the plant cell to an effective concentration of an inhibitor herbicide.

TAL-effector mediated herbicide tolerance

Materials and methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; 'TALENS') are provided.

TAL effector-mediated DNA modification

Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; 'TALENS') are provided.

Tal effector-mediated DNA modification

The present invention is directed to an isolated protein or polypeptide which elicits a hypersensitive response in plants as well as an isolated DNA molecule which encodes the hypersensitive response eliciting protein or polypeptide. This isolated protein or polypeptide and the isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. This can be achieved by applying the hypersensitive response elicitor protein or polypeptide in a non-infectious form to plants or plant seeds under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds. Alternatively, transgenic plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response elicitor protein or polypeptide can be provided and the transgenic plants or plants resulting from the transgenic plant seeds are grown under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.

Hypersensitive response elicitor from Erwinia amylovora, its use, and encoding gene

One aspect of the present invention relates to a DNA construct that contains a first DNA molecule encoding a functional type III secretion system, a promoter, and a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. The second DNA molecule is operably coupled to the promoter so that upon introduction of the DNA construct into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Another aspect of the present invention relates to a system that includes (i) a first DNA construct having a first DNA molecule encoding a functional type III secretion system and (ii) a second DNA construct having a promoter operably coupled to a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. Upon introduction of the first and second DNA constructs into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Methods of isolating a protein or polypeptide and identifying a gene encoding a potential effector protein or polypeptide are also disclosed.

Recombinant constructs and systems for secretion of proteins via type III secretion systems

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Ithaca, NY, United States of America

Adam J Bogdanove