Dsm IP Assets B.v.

Adam G Lawrence

Manoj N Kumar

Viktor Marius Boer

Marco Alexander Van Den Berg

Cornelis Maria Jacobus Sagt

Priscilla Zwartjens

Liang Wu

Bernard Meijrink

Nicolette Jasmijn Broers

Celine ViArouge

Joshua Truehart

Joshua Trueheart

Viktor Boer

Martin Lehmann

Manoj Kumar

The present disclosure relates to a novel method, expression vectors, and host cells for producing nicotinamide riboside by regulating the pathways that lead to the production of nicotinamide riboside.

Microbial production of nicotamide riboside

The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genusin a suitable fermentation medium at a temperature of about 29&#xb0; C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.

Diterpene production in Yarrowia

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Arlington, MA, United States of America

Adam G Lawrence