Visby Medical, Inc.

Click Diagnostics, Inc.

Leland Stanford Junior University

Adam De La Zerda

Gregory Loney

David Swenson

Boris Andreyev

Victor Briones

Brian Ciopyk

Steven Chu

Colin Kelly

Helen Huang

Jesus Ching

Keith Edward Moravick

Jonathan Hong

Ryan Cena

Alan Baldwin

Kenneth I Li

Gary K Schoolnik

Kevin M Limtao

Hamilton Roger Tang

Valeria Revilla

Adrienne C Lam

Bryan Knysh

Sanjiv Sam Gambhir

Shaunak Roy

Moritz F Kircher

Yonatan Winetraub

Cristina Zavaleta

Phoebe Cao

Edwin Yuan

Caroline Yu

Itamar Terem

Gary Schoolnik

Orly Liba

Elliott Sorelle

Milan S Gambhir

Jennifer Albrecht

Rajinder K Bhatia

Danielle McSheery

Edward Biba

Jesse Jokerst

Steve Chu

Alignment of a 2D image to a corresponding 3D image is provided by writing a pattern into a 3D sample. The pattern is at known positions in the 3D image, and provides visible reference features in the 2D image. This permits accurate determination of the plane in the 3D image that corresponds to the 2D image.

High resolution alignment of 3D imaging with 2D imaging

An apparatus includes a substrate, a first heating element, and a second heating element. The substrate includes a first portion, a second portion, and a third portion that is between the first portion and the second portion. The first portion is characterized by a first thermal conductivity, the second portion is characterized by a second thermal conductivity, and the third portion is characterized by a third thermal conductivity. The third thermal conductivity is less than the first thermal conductivity and the second thermal conductivity. The first heating element is coupled to the first portion of the substrate, and is configured to produce a first thermal output. The second heating element is coupled to the second portion of the substrate, and configured to produce a second thermal output. The second thermal output is different from the first thermal output.

Printed circuit board heater for an amplification module

A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.

Devices and methods for molecular diagnostic testing

A method includes coupling a molecular diagnostic test device to a power source. A biological sample is conveyed into a sample preparation module. The device is then actuated by only a single action to cause the device to perform the following functions without further user action. First, the device heats the sample via a heater of the sample preparation module to lyse a portion of the sample. Second, the device conveys the lysed sample to an amplification module and heats the sample within a reaction volume of the amplification module to amplify a nucleic acid thereby producing an output solution containing a target amplicon. The device then reacts, within a detection module, each of (i) the output solution and (ii) a reagent formulated to produce a signal that indicates a presence of the target amplicon within the output solution. A result associated with the signal is then read.

Portable molecular diagnostic device and methods for the detection of target viruses

Disclosed herein are methods and devices for preparing a sample of nucleic acid molecules from a biological sample. The methods and devices may perform similarly to or better than standard sample preparation methods. The nucleic acid molecules prepared using the methods and devices provided herein may be utilized for downstream applications, including polymerase chain reaction (PCR).

Devices and methods for nucleic acid extraction

Cartridge-based thermocyclers can include cartridges that are configured to move a fluid between distinct chambers. In some cases, the cartridge-based thermocyclers can be used for thermocycling a sample fluid comprising a deoxyribonucleic acid (DNA) target to perform polymerase chain reaction (PCR). Individual chambers can be heated, cooled, and/or compressed to mix fluid within the chamber or to propel fluid in the chamber into another chamber. The cartridges can have a laminate construction. The cartridges can be configured to enable multiplexed thermocycling and/or detection.

Cartridge-based thermocycler

A composition includes a plurality of gold nanoparticles each having at least one surface. The gold nanoparticles have an average length of at least about 90 nm and an average width of at least about 25 nm.

High-resolution optical molecular imaging systems, compositions, and methods

An apparatus for detecting circulating tumor cells is provided. A support system for mounting on a patient is provided. An imaging system is attached to the support system. The imaging system comprises a transmitter and receiver system for generating and transmitting signals into the patient and receiving signals from the patient and a controller. The controller comprises a processor and computer readable media. The computer readable media comprises computer readable code for transmitting signals into the patient, computer readable code for receiving signals from the patent, and computer readable code for providing in vivo circulating tumor cell imaging agent data from the received signals from the patient.

Wearable ultrasonic device for circulating tumor cell detection

A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.

Devices and methods for the detection of molecules using a flow cell

Growing community of inventors

Palo Alto, CA, United States of America

Adam De La Zerda