Illumina Cambridge Limited

Adam Culley

Xiaohai Liu

Antoine Francais

Elena Cressina

Angelica Mariani

Xiaolin Wu

Anno Koetje

Embodiments of the present disclosure relate to nucleotide with acetal 3'-OH blocking groups. Also provided herein are methods of using fully functionalized nucleotides containing the 3′ acetal blocking group for sequencing applications.

Methods of sequencing using nucleotides with 3' acetal blocking group

Embodiments of the present disclosure relate to nucleotide molecules with a 3' AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

Nucleotides with a 3' AOM blocking group

Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3' AOM blocking group. Also provided herein are kits related to such methods.

Methods of preparing growing polynucleotides using nucleotides with 3' AOM blocking group

Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with acetal 3'-OH blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and the uses of fully functionalized nucleotides containing the 3&#x2032; acetal blocking group for sequencing applications.

Nucleosides and nucleotides with 3' acetal blocking group

Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3' AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.

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Cambridge, United Kingdom

Adam Culley