Life Technologies Corporation

Applied Biosystems, LLC

Adam Broomer

Caifu Chen

Kelly Li

Andreas Tobler

Kenneth J Livak

Mark R Andersen

David Keys

David N Keys, Jr

This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof.

Karyotyping assay

The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.

Encoding and decoding reactions for determining target molecules

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Carlsbad, CA, United States of America

Adam Broomer