Amazon Technologies, Inc.

Seattle Children's Hospital

Adam Brent Johnson

Adam Lloyd Days

Michael C Jensen

Yashar Dehkharghani Fakhari

Mehul Gordhanbhai Sutariya

Jinesh Udaykumar Vora

Llewellyn James Mason

Min Chong

Francisco Jose Peredo Noguez

Margaret E McCanner

Brian Chang

John Riley Lynch

Craig Wesley Howard

Jorge Peixoto Vasquez

Paul William Berg

Jonathan Paul Sillito

Jonathan Gilbert Gordon Tams

David Paul Willis

Embodiments of the methods and compositions provided herein relate to chimeric antigen receptors (CARs) that specifically bind to B7H3. Some embodiments relate to cell-based immunotherapy targeting tumors, such as tumors comprising B7H3+ cells.

Methods and compositions comprising B7H3 chimeric antigen receptors

The present invention provides genetic tags operably linked to transgenes. The expression of the genetic tag allows identification, detection, selection, and ablation of cells expressing the transgene and the genetic tag. In some alternatives the genetically modified host cell comprises a transgene comprising a polynucleotide coding for a chimeric antigen receptor comprising a ligand binding domain, a polynucleotide comprising a spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain and a polynucleotide coding for a genetic tag. In some alternatives the genetically modified host cell comprises a transgene comprising a polynucleotide coding for a chimeric antigen receptor comprising a ligand binding domain, a polynucleotide comprising a spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain and a polynucleotide coding for a genetic tag, and wherein the polypeptide further comprises a flexible linker comprising amino acids GGGSGGGS (SEQ ID NO:45). Pharmaceutical formulations produced by the method, and methods of using the same, are also described.

Transgene genetic tags and methods of use

Techniques for information passing for content-based source selection via DNS are described. A domain name specified in a DNS lookup request includes one or more hostname and/or subdomain values that are generated based on obfuscating a path of the object that is ultimately to be sought by the client. The authoritative DNS server can identify the desired object, and select a particular network address for the lookup response in a more intelligent manner based on characteristics of the sought object, server location availabilities and characteristics, client device characteristics, etc.

Information passing for content-based source selection via DNS

The present application relates to fusion proteins, chimeric antigen bearing cells expressing fusion proteins and compositions comprising chimeric antigen bearing cells expressing fusion proteins. The application further relates to methods of using the fusion proteins, cells and compositions for modulating an immune response.

Tumor-specific IFNA secretion by car T-cells to reprogram the solid tumor microenvironment

An interactive user interface is disclosed that enables users to efficiently access and/or submit component-specific reviews of components of a product. The user interface displays an image of the product, and displays region identifiers that identify image regions corresponding to product components that are separately reviewable. When a user selects a particular region, the user interface may display component-specific reviews of the corresponding component, and/or may display an option to submit a component-specific review of the component. The user interface may be included in an interactive catalog page, such as a web page.

System with interactive user interface for efficiently accessing component-level reviews

The present invention provides genetic tags operably linked to transgenes. The expression of the genetic tag allows identification, detection, selection, and ablation of cells expressing the transgene and the genetic tag. In some alternatives the genetically modified host cell comprises a transgene comprising a polynucleotide coding for a chimeric antigen receptor comprising a ligand binding domain, a polynucleotide comprising a spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain and a polynucleotide coding for a genetic tag. In some alternatives the genetically modified host cell comprises a transgene comprising a polynucleotide coding for a chimeric antigen receptor comprising a ligand binding domain, a polynucleotide comprising a spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain and a polynucleotide coding for a genetic tag, and wherein the polypeptide further comprises a flexible linker comprising amino acids GGGSGGGS (SEQ ID NO: 45). Pharmaceutical formulations produced by the method, and methods of using the same, are also described.

Disclosed are various embodiments for assigning user interface widgets to page slots associated with a page template. In response to a request to generate a content page, content placement requests are then received from user interface widgets. The page slots are associated with a placement value indicating a value of the page slot relative to other page slots in the page template. The highest ranked user interface widgets according to an effectiveness metric or placement score are scheduled for placement in the content page.

Assigning slots to user interface elements

Methods, devices and program products are provided which comprise the under control of one or more computer systems configured with specific executable instructions. The methods, devices and program products provide an image of an item for display, and the item is comprised of a plurality of components. The methods, devices and program products receive a selection of a component of interest from the plurality of components of the item, and obtain a component-level rating associated with the component of interest.

Component-level rating system and method

A system and a method for the management of addressable network resources (e.g., Web pages, electronic documents accessed via a Uniform Resource Identifier, etc.) within a network are provided. Specifically, embodiments of addressable network resource selection systems and methods are disclosed for managing the analysis and identification of optimal links between addressable network resources. A resource selection component enables pruning of undesirable pages on the basis of various usage metrics, including user depart rate. The resource selection component may then identify optimal linking strategies by determining one or more linking scores for remaining pages based on any of a number of different page usage metrics. In other embodiments, addressable network resource selection systems and methods may weight linking scores to emphasize desired outcomes, such as encouraging exploration of a network-based site or encouraging high aggregate sales figures.

Addressable network resource selection management

Disclosed are various embodiments for assigning user interface widgets to page slots associated with a page pipeline. In response to a request to generate a content page, content placement requests are then received from user interface widgets. The page slots are associated with a placement value indicating a value of the page slot relative to other page slots in the page template and can be weighted based upon their location in a page pipeline. The highest ranked user interface widgets according to an effectiveness metric or placement score are scheduled for placement in the content page.

Assigning slots to content in a pipeline

Methods, systems, and computer-readable media for automated page generation using value-based recommendations are disclosed. A plurality of content recommendations are determined for a web page using one or more recommenders. Individual ones of the content recommendations are assigned to individual ones of a plurality of interaction elements. Respective effectiveness values for individual ones of the interaction elements are determined based at least in part on the individual ones of the content recommendations assigned to the individual ones of the interaction elements. Based at least in part on the respective effectiveness values for the individual ones of the interaction elements, individual ones of the interaction elements are assigned to respective portions of the web page. The web page is generated based at least in part on the individual ones of the interaction elements assigned to the respective portions of the web page.

Automated page generation using value-based recommendations

Technologies are described herein for delegating requests in a service-oriented architecture (SOA) environment. A calling service in the SOA environment can delegate the task of obtaining data used in a computation to a called service that performs the computation. In order to delegate, the calling service may provide a request specification that defines a mechanism by which the called service can obtain the data. For example, the request specification might define a request to another service in the SOA for obtaining the data. The calling service might also provide an authentication ticket that can be utilized to confirm that the calling service has delegated the task to the called service. The called service can utilize the request specification and the authorization ticket to obtain the data. The obtained data may then be used to perform a computation. The results of the computation may then be provided to the calling service.

Growing community of inventors

Seatte, WA, United States of America

Adam Brent Johnson