Degussa Aktiengesellschaft

Evonik Roehm Gmbh

Other

Degussa - Huls Ag

Evonik Degussa Gmbh

Evonik Rohm Gmbh

National University of Ireland

Achim Marx

Walter Pfefferle

Mike Farwick

Thomas Hermann

Brigitte Bathe

Bettina Mockel

Bettina Möckel

Klaus Huthmacher

Caroline Kreutzer

Bettina M&ouml;ckel

Thomas Haas

Steffen Schaffer

Lothar Eggeling

Mechthild Rieping

Bettina Moeckel

Jörn Kalinowski

Natalie Schischka

Alfred Pühler

Markus Poetter

Liv Reinecke

Georg Thierbach

Hermann Sahm

Thomas Tacke

Michael Binder

Hermann Siegert

Alexander May

Olivier Zehnacker

Anja Thiessenhusen

Stefan Buchholz

Ashling McCormack

Kevin Burke

Christine Bastuck

Alfred P&uuml;hler

Cliona Stapelton

Markus Pötter

Eva Maria Wittmann

Tim Koehler

J&ouml;rn Kalinowski

IIona Walger

Ulrich Becker

Alexander Schraven

Muriel Merkamm

Albert De Graaf

Michael Buchholz

Bettina Hädrich

Birgit Alber

Indra Schr&ouml;der

Mike Garwick

Georg Fuchs

Michael O'Donohue

L K Dunican

Markus Buchhaupt

Cliona Stapleton

Klaus Erich Uffmann

Armel Guyonvarch

Eva-Maria Wittmann

L K Prof Dunican

Ashling Mccormback

The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.

Recombinant cell producing 2-hydroxyisobutyric acid

The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

Process for preparing methacrylic acid or methacrylic esters

The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.

Use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase

A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R, Rand R, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.

Method for the production of free carboxylic acids

Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

Process for the preparation of l-amino acids

The invention related to an isolated nucleic acid consisting of a fragment of the polynucleotide ofcitA gene or a fragment of the full complement of the polynucleotide of thecitA gene wherein said fragment is at least 30 consecutive nucleotide in length, and the vector and host cell harboring these nucleic acids.

[object Object]

A process for the production of an L-amino acid wherein coryneform bacteria (e.g.) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.

Process for the production of L-amino acids by fermentation using coryneform bacteria

The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group

Nucleotide sequences coding for the citE gene

The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.

Nucleotide sequences which code for the ccpA2 gene

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

Polynucleotide sequences which encode the zwa1 gene

Process for the production of L-amino acids by fermentation using coryneform bacteria with an attenuated mqo gene

The invention relates to an isolated polynucleotide fromhaving a polynucleotide sequence which codes for the carbon starvation protein A (cstA) gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in enhanced form and a vector which carries at least the cstA gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Nucleotide sequences which code for the cstA gene

Nucleotide sequences which code for the menE gene

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Nucleotide sequences which code for the citB gene

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the gorA gene, and a host-vector system having a coryneform host bacterium in which the gorA gene is present in attenuated form and a vector which carries at least the gorA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Nucleotide sequences which code for the gorA gene

Growing community of inventors

Bielefeld, Germany

Achim Marx