Caribbean Microparticles Corporation

Flow Cytometry Standards Corporation

Becton Dickinson and Company Limited

Other

Magnetic Activated Particle Sorting, Inc.

Abraham Schwartz

Joel L Williams

Robert D Stevens

Gary Woodward

Jane C Graper

Robert F Vogt

Emma Fernandez-Repollet

Alan D Hetzel

Jay H Schwartz

An adjustable LED lighting fixture which accommodates LED lights of varying lengths. The LED fixture has a central body element, and two movable end elements movably attached to two opposing sides of the central body element and extend in a direction normal to a length of the fixture. The two end elements face each other with LED pin sockets facing each other whereby an LED tube light is able to be operationally inserted into the LED pin sockets. Two slidable elements are attached to the end elements and extending in a direction in a direction along the length of the fixture and toward the other of the end elements and through a retention element. The slidable elements permit the expansion and contraction of the length of the LED fixture, by sliding relative movement toward and away from the other of the end elements, to hold LED light tubes of varying lengths.

Adjustable size LED fixture

A heat transfer system including mechanical and electrical components for use with a mechanical air conditioning system to enable the system to efficiently reject heat to a water source, such as a pool or spa while simultaneously cooling an interior space. The air conditioning system incorporates three primary heat transfer coils in a mechanical refrigeration cycle to provide comfort cooling to an interior space while rejecting heat to either the atmosphere or a water source, such as a swimming pool. In an alternate heat pump embodiment, the system is capable of operating in an additional mode to absorb heat from the atmosphere and reject heat to the interior space.

Combination air conditioning system and water heater

A coded hidden identification is provided for a selected printable item by providing a coded printing composition based on adding to and substantially uniformly mixing a selected number of populations of microparticles with a printing composition, said microparticles being normally invisible to the human eye and each such population having a group of microparticles of similar size, shape or color to form a combination of highly uniform and unique properties, the printing composition adapted for use in a printing machine, such as a photocopy or laser printing machine cartridge, and printing items with the coded printing composition so as to transfer the microparticles to the item. The microparticles may be characterized by a number of detectable properties, or a combination thereof, which are not detectable by the human eye to provide the coded hidden identification. The microparticles may contain, for example, a fluorescent dye and be made to fluoresce to reveal the identification, or they may be of a form such that magnification may be used to reveal the hidden identification.

Composition, method and apparatus for providing a coded hidden

A system for rapid microbead calibration of a flow cytometer including a suspension of quantitative fluorescent microbead standards and analytical software. The software is used to take information on the microbead suspension from a flow cytometer and analyze data, smooth curves, calculate new parameters and notify of expiration of the system.

Automated system for performance analysis and fluorescence quantitation

A method of setting up a flow cytometer using microbeads fluorescing at a plurality of wavelengths, control cells for each of said wavelengths, certified blank microbeads. The blank microbeads are run and the PMT voltages are adjusted so that the blank microbeads are on scale on the flow cytometer. The control cells are run and the photomultiplier tube voltages are adjusted so that the single population falls in selected channels. The fluorescent microbeads are run on the flow cytometer and the Target Channels for the flow cytometer are determined.

Method of setting up a flow cytometer

A kit of highly uniform size microbead standards for flow cytometer alignment, compensation, and/or calibration, comprising a blank microbead population and/or an auto-fluorescent microbead population, together with two or more series of calibrated microbead populations labeled with fluorescent dye(s) which (i) prior to fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of the blank and/or autofluorescent microbead population, and (ii) after fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of fluorescently labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and/or calibrate a flow cytometer so as to make measurements on samples comparable and independent of the specific instrument and instrument settings.

Method of aligning, compensating, and calibrating a flow cytometer for

A qualitative and quantitative method of determining the fluorescence threshold of an instrument and of determining whether the instrument is sensitive enough to measure the autofluorescence of a sample. The method utilizes non-fluorescent particles such a microbeads which are run on a flow cytometer. The peak channel position of the microbeads is used as the fluorescence threshold.

Method of use of non-fluorescent particles to determine fluorescence

The use of a combination of calibrated microbead populations with one or more calibrated biological cell populations to correct calibration of a flow cytometer for size and fluorescence intensity determinations of biological cell samples. The use of calibrated biological cells permits correction for factors related to the instrument and calibration microbeads so long as the excitation and emission spectra of the calibration microbeads, the calibration cells and the cell samples are all the same, respectively.

Method for corrective calibration of a flow cytometry using a mixture of

A flow cytometer reference standard containing a highly uniform microbead population, wherein each microbead has the same multiple fluorescent labels as the other microbeads and as the samples to be measured. Target conditions are set for the sample after alignment of the flow cytometer. The microbead reference standard is used to determine peak channels (target channels) for each parameter. The target conditions may be reestablished for later uses of the flow cytometer with the same type of samples by using the microbead reference standard to adjust the flow cytometer so that the microbead peak channels fall in the originally determined target channels.

Microbead reference standard and method of adjusting a flow cytometer to

A method for calibrating a flow cytometer or fluorescence microscope in terms of number of binding antibodies as a function of fluorescence intensity value measured on the flow cytometer or fluorescence microscope, and subsequent measuring of a sample to which the antibodies are bindable. Also disclosed is a microbead calibration kit for carrying out the calibration method of the invention. The disclosed calibration methodology provides a direct relationship between instrument response and numbers of binding antibodies, independent of the fluorochrome employed to label the samples being measured. The method has utility in monitoring the status of an antigenic cellular condition in which the number of antibodies binding to successively obtained cellular samples is determined, to establish the progressionary character of the antigenic cellular condition, in a host from which the cellular samples are taken.

Method of calibrating a flow cytometer or fluorescence microscope for

A method of calibrating a flow cytometer or fluorescent microscope is based on a set of highly uniform microbeads associated with a fluorescent dye in such a way that the microbeads have the same excitation and emission spectral properties as the samples which are to be measured. The calibration values of the microbeads are plotted against the relative fluorescence intensity peak channel or intensity reading for each microbead in the set. From this calibration plot, the relative fluorescence intensity peak channel or intensity reading of the sample is translated into equivalent soluble fluorescent dye molecules per sample particle. The calibration values of the standard microbeads are determined against solutions of the dyes. In cases where the background scatter of the bulk microbeads suspensions is too high for a direct determination against the solutions, a different set of microbeads with low background scatter is calibrated against the dye solutions and used to make an initial calibration of a flow cytometer or fluorescent microscope, which in turn, is used to calibrate the uniform microbead standards. A novel method of making the microbead standards is also disclosed.

Method of calibrating a fluorescent microscope using fluorescent

A kit of highly uniform microbead standards for flow cytometer alignment, compensation, and calibration, comprising a blank and/or auto-fluorescent microbead population, together with two or more series of calibrated microbead populations which match the fluorescence spectra of labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and calibrate a flow cytometer so as to make measurements on corresponding samples comparable and independent of the specific instrument and instrument settings.

Method of compensating and calibrating a flow cytometer, and microbead

The synthesis, composition and use of particles exemplified by microbeads of uniform size and character, with covalently bound biological molecules for biological simulation is disclosed.

Composition, synthesis and use of simulated cells

A method of calibrating a flow cytometer or fluorescent microscope is based on a set of highly uniform microbeads associated with a fluorescent dye in such a way that the microbeads have the same excitation and emission spectral properties as the samples which are to be measured. The calibration values of the microbeads are plotted against the relative fluorescence intensity peak channel for each microbead in the set. From this calibration plot, the relative fluorescence intensity peak channel of the sample is translated into equivalent soluble fluorescent dye molecules per sample particle. The calibration values of the standard microbeads are determined against solutions of the dyes. In cases where the background scatter of the bulk microbeads suspensions is too high for a direct determination against the solutions, a different set of microbeads with low background scatter is calibrated against the dye solutions and used to make an initial calibration of a flow cytometer or fluorescent microscope, which in turn, is used to calibrate the uniform microbead standards. A novel method of making the microbead standards is also disclosed.

Fluorescent calibration microbeads simulating stained cells

A method of calibrating a flow cytometer is based on a set of highly uniform microbeads associated with a fluorescent dye in such a way that the microbeads have the same excitation and emission spectral properties as the samples which are to be measured. The calibration values of the microbeads are plotted against the relative fluorescence intensity peak channel for each microbead in the set. From this calibration plot, the relative fluorescence intensity peak channel of the sample is translated into equivalent soluble fluorescent dye molecules per sample particle. The calibration values of the standard microbeads are determined against solutions of the dyes. In cases where the background scatter of the bulk microbeads suspensions is too high for a direct determination against the solutions, a different set of microbeads with low background scatter is calibrated against the dye solutions and used to make an initial calibration of the flow cytometer, which in turn, is used to calibrate the uniform microbead standards. A novel method of making the microbead standards is also disclosed.

Growing community of inventors

Durham, NC, United States of America

Abraham Schwartz