Harvard College

Children's Medical Center Corporation

Abhishek Jain

Donald Elliot Ingber

Andries D Van Der Meer

Daniel Levner

Christopher David Hinojosa

Riccardo Barrile

Andrew L Frelinger, Iii

Daniel C Leslie

Anna Waterhouse

Alan David Michelson

Marjon Zamani

Marinke Van Der Helm

Mike Super

Andries Van Der Meer

Christopher David Hinjosa

Iii Andrew L Frelinger

Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

Fluidic device for quantifying the dynamic permeability and hydraulic conductivity of living tissue layers

A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

Methods, systems, and compositions for determining blood clot formation, and uses thereof

In accord with one aspect, a microfluidic coagulation assessment device defining a plurality of microchannels is provided, wherein a blood sample is driven through the microchannels at a substantially constant flow rate and a controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (Tpg) at which a second pressure value is about twice the determined first pressure value, and a second time (Tpf) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom. In another aspect, the blood sample is driven through the microchannels at a substantially constant pressure and a controller is configured to, in combination with a timer and a flow sensing device make the determination based on flow rate.

Microfluidic device for real-time clinical monitoring and quantitative assessment of whole blood coagulation

Growing community of inventors

Roslindale, MA, United States of America

Abhishek Jain