Plexxikon Inc.

Inscripta, Inc.

Eleszto Genetika, Inc.

Abhinav Kumar

Prabha N Ibrahim

Jiazhong Zhang

Chao Zhang

Brian L West

Dean R Artis

Marika Nespi

Ryan Bremer

Shumeye Mamo

Rebecca Zuckerman

Clarence R Hurt

Heike I Krupka

Gaston G Habets

Yong-Liang Zhu

Yong Zhu

Amanda Reider Apel

Szilvia Varszegi

Lorand Szabo

Bela Krisztian Szamecz

Drew Fraser Thacker

Karolina Kalbarczyk

Richard Dean Artis

Helke Krupka

The present disclosure relates to synthetic biology and, in particular, the bioproduction of bakuchiol, and engineered enzymes for producing the same.

Engineered enzymes and bioproduction of bakuchiol

Disclosed herein are microorganism and methods that can be used for the synthesis of cannabigerolic acid (CBGA) and cannabinoids. The methods disclosed can be used to produce CBGA, Δ-tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabichromenic acid (CBCA), Δ-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabichromene (CBC). Enzymes useful for the synthesis of CBGA and cannabinoids, include but are not limited to acyl activating enzyme (AAE1), polyketide synthase (PKS), olivetolic acid cyclase (OAC), prenyltransferase (PT), THCA synthase (THCAS), CBDA synthase (CBDAS), CBCA synthase (CBCAS), HMG-Co reductase (HMG1), and/or farnesyl pyrophosphate synthetase (ERG20). The microorganisms can also have one or more genes disrupted, such as gene that that controls beta oxidation of long chain fatty acids.

Microorganisms and methods for the fermentation of cannabinoids

Compounds active on Ret are described, as well as methods of using such compounds. Also described are crystal structures of Ret surrogates that were determined using X-ray crystallography. The use of such Ret surrogate crystals and structural information can, for example, be used for identifying molecular scaffolds and for developing ligands that bind to and modulate Ret and for identifying improved ligands based on known ligands.

Compounds and methods for development of Ret modulators

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Pleasant Hill, CA, United States of America

Abhinav Kumar