Ge Healthcare Bio-Sciences Ab

Amersham Biosciences Ab

Abhijit Mazumder

Richard D Shippy

David Dorris

Chang-Gong Liu

Robert Elghanian

Charles K Brush

Kenneth Luehrsen

Vineet Gupta

W Travis Johnson

Carl Yamashiro

David R Fermin

Gretchen Kiser

Zhijie John Gu

Changming Li

Ekaterina Pestova

Krishnamurthy Jayaraman

David G Wang

Ramesh Ramakrishnan

Arkadiy Silbergleyt

Todd Tuggle

Buena Chui

Handy Yowanto

Disclosed herein are methods of selecting probes to target nucleic acid sequences, methods of making oligonucleotide arrays comprising such probes, and methods of using such arrays. Also, described herein are oligonucleotide arrays comprising probes selected by a method of the invention.

Ratio-based oligonucleotide probe selection

This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink&#x2122;, eSensor&#x2122;, multiplex arrays with cartridges etc., all described herein.

P450 single nucleotide polymorphism biochip analysis

Methods and devices for detecting nucleic acid and protein targets on hydrogel microarrays are disclosed. Fluorophores are incorporated into the targets and detected. A linear correlation between target concentration and signal amplitude is maintained through the elimination of active enzyme amplification.

Hydrogel-based microarray signal amplification methods and devices therefor

Growing community of inventors

Buffalo Grove, IL, United States of America

Abhijit Mazumder