University of South Florida

Abdur Rub Abdur Rahman

Shekhar Bhansali

Sunny Kedia

An electrical impedance sensing device which integrates multielectrode automated impedance spectroscopy capability with automatic parameter extraction and data analysis to create an automated cell behavior monitoring system. The device comprises radial electrodes and an out-of-plane counter electrode. Quantitative impedance data provided information on cell adhesion, spreading, proliferation and detachment due to cell cycle processes as well as cell-drug interaction, with spatio-temporal resolution. The resulting dataset is processed for impedance distribution and used to characterize cellular motion, morphology, electrochemical and dielectric properties. Also, a method is described for studying cell-cell and cell-matrix interactions, determining electrical characteristics of cell layers, and identifying specific impedance parameters for cancer screening, drug screening, bacterial growth monitoring, organ transplant compatibility, and cell-drug interaction among other applications.

Impedance spectroscopy-based cellular analysis device

The present invention illustrates a bulk silicon etching technique that yields straight sidewalls, through wafer structures in very short times using standard silicon wet etching techniques. The method of the present invention employs selective porous silicon formation and dissolution to create high aspect ratio structures with straight sidewalls for through wafer MEMS processing.

Growing community of inventors

Tampa, FL, United States of America

Abdur Rub Abdur Rahman