Ultivue, Inc.

Abdul Majeed Mohammed

Michael J Natan

Mael Manesse

Mark Norman Bobrow

Mack J Schermer

Xi Chen

Stephanie Rae Hennek

Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

Methods and compositions for sequentially detecting targets

The present application provides certain advantageous ways of conducting multiplexed imaging.

Methods for multiplex imaging using labeled nucleic acid imaging agents

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Cambridge, MA, United States of America

Abdul Majeed Mohammed