Biomerieux, Inc.

Centre National De La Recherche Scientifique

Université Claude Bernard

Alpol Cosmetique

Abdelhamid Elaissari

Bernard F Mandrand

Christian Pichot

Thierry Delair

Jérôme Bibette

Olivier Mondain-Monval

Ahmed Arkis

Claude Mabilat

Hatem Fessi

Eric Bosc

Doran Spencer

Marie-Helene Charles

Philippe Cros

Marc Rodrigue

Franck Montagne

Lise Santoro

Florence Sauzedde

Lionel Ripoll

Sabrina Etheve

Claire Bordes

Sébastien Braconnot

The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z', which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, &#x3b1;-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell

Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses

The invention relates to composite particles comprising a hydrophobic polymer core and inorganic nanoparticles. Said hydrophobic polymer forms a polymer matrix inside which the inorganic nanoparticles are stabilized and distributed in a relatively homogenous manner. Said particles are at least partially surrounded by an amphophilic copolymer comprising a hydrophobic part and a hydrophilic part, said hydrophobic part being at least partially immobilized in the polymer matrix. The inventor also relates to the preparation method thereof, the conjugates comprising such particles and a ligand and the use of same in reactants and therapeutic compositions.

Composite particles, derived conjugates, preparation method and applications

The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z', capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, &#x3b1;-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising such a complex or colloidal particles.

The invention concerns composite nanospheres having a diameter ranging between about 50 and 1000 nm plus or minus 5%, preferably between about 100 and 500 nm plus or minus 5% and advantageously between 100 and 200 nm plus or minus 5%, and comprising an essentially liquid core consisting of an organic phase and inorganic nanoparticles, distributed inside the organic phase, and a skin consisting of at least a hydrophilic polymer derived from the polymerisation of at least one water soluble monomer, in particular N-alkylacrylamide or a N-N-dialkylacrylamide; conjugates derived from said nanospheres; their preparation methods and their uses.

Composite nanospheres and their conjugates with biomolecules

The invention concerns composite nanospheres having a diameter ranging between about 50 and 1000 nm plus or minus 5%, preferably between about 100 and 500 nm plus or minus 5% and advantageously between 100 and 200 nm plus or minus 5%, and comprising an essentially liquid core consisting of an organic phase and inorganic nanoparticles, distributed inside the organic phase, and a skin consisting of at least a hydrophilic polymer derived from the polymerisation of at least one water soluble monomer, in particular N-alkylacrylamide or a N&#x2014;N-dialkylacrylamide; conjugates derived from said nanospheres; their preparation methods and their uses.

A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45&deg; C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10 M is achieved.

Nucleic acid isolation

The invention concerns magnetic and heat-sensitive particles having a predetermined size between 0.05 and 10 &mgr;m, each particle comprising: an inner composite core consisting of a solid organic or inorganic particle, containing within it a magnetic filler; an outer coati based on a polymer capable of interacting with at least a biological molecule, the outer polymer is heat-sensitive and has a predetermined low critical solubility temperature (LCST) ranging between 10 and 100&deg; C. and preferably between 20 and 60&deg; C. The invention also concerns methods for obtaining said particles and their uses. The particles are characterized in that there is an intermediate layer between the inner core and the outer layer isolating said inner core magnetic filler with respect to said functionalized outer layer. Said invention is particularly useful for separating proteins and/or nucleic acids.

Magnetic particles, method for obtaining same and uses for separating molecules

Superparamagnetic monodisperse particles

Nucleotide sequence detection with signal amplification

Growing community of inventors

Lyons, France

Abdelhamid Elaissari