University of California

University of Hawai'i

Aaron T Ohta

Ming C Wu

Pei-Yu E Chiou

Tom F Lue

Justin K Valley

Maurice Marcel Garcia

Ryan Miyamoto

Wayne A Shiroma

Grant S Shiroma

Blaine T Murakami

Michael Tamamoto

Ming-Chiang Wu

Pei Yu Chiou

Arash Jamshidi

Steven L Neale

Hsan-Yin Hsu

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The preferred OET-DEP devices generally comprise a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a localized virtual electrode with a DEP electric field gradient of selected intensity along with input and a plurality of output fluidic channels. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles/cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

Methods and devices for sorting cells and other biological particulates

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 &#x3bc;m or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or group of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

Optoelectronic tweezers for microparticle and cell manipulation

Described herein are single-sided lateral-field optoelectronic tweezers (LOET) devices which use photosensitive electrode arrays to create optically-induced dielectrophoretic forces in an electric field that is parallel to the plane of the device. In addition, phototransistor-based optoelectronic tweezers (PhOET) devices are described that allow for optoelectronic tweezers (OET) operation in high-conductivity physiological buffer and cell culture media.

Single-sided lateral-field and phototransistor-based optoelectronic tweezers

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 &#x3bc;m or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

A high-directivity transponder system uses a dual system of a retrodirective array transmitting a data signal peak toward an interrogator source, and a self-null-steering array transmitting a null toward the interrogator source and a jamming signal elsewhere, resulting in high S/N reception at the interrogator source and avoidance of interception. Integrating modulators would allow each array to transmit different data while the spectra of the transmitted signals are identical, thus disabling interception. The system enables secure point-to-point communications and can be used for short-distance wireless data transmission systems such as wireless LAN and RFID servers. One mobile may send a broadcast interrogator signal, causing the other mobile devices to send a reply signal back to the interrogating mobile device only in the direction of the interrogating mobile device, such that each of the other mobile devices identifies its position to only the interrogating mobile device in response to its broadcast interrogator signal. As another aspect, self-steering signal transmission is employed for randomly oriented satellites using circularly polarized, two-dimensional retrodirective arrays. Quadruple subharmonic mixing is used as an effective means of achieving phase conjugation when a high-frequency LO is not feasible or inapplicable. These features may be used for small-satellite communications, secure tactical communications, search and rescue, enemy location fixing and tracking, UAV command and control, forest fire detection, marine-based tracking, and many other applications requiring secure communications with high signal directivity.

Microwave self-phasing antenna arrays for secure data transmission and satellite network crosslinks

A high-directivity transponder system uses a dual system of a retrodirective array transmitting a data signal peak toward an interrogator source, and a self-null-steering array transmitting a null toward the interrogator source and a jamming signal elsewhere, resulting in high S/N reception at the interrogator source and avoidance of interception. Integrating modulators would allow each array to transmit different data while the spectra of the transmitted signals are identical, thus disabling interception. The system enables secure point-to-point communications and can be used for short-distance wireless data transmission systems such as wireless LAN and RFID servers. As another aspect, self-steering signal transmission is employed for randomly oriented satellites using circularly polarized, two-dimensional retrodirective arrays. Quadruple subharmonic mixing is used as an effective means of achieving phase conjugation when a high-frequency LO is not feasible or inapplicable. These features may be used for small-satellite communications, secure tactical communications, search and rescue, enemy location fixing and tracking, UAV command and control, forest fire detection, marine-based tracking, and many other applications requiring secure communications with high signal directivity.

Growing community of inventors

Berkeley, CA, United States of America

Aaron T Ohta