Zymergen Inc.

Aaron Miller

Kedar Patel

Shawn Szyjka

Philip Weyman

Sara Da Luz Areosa Cleto

Sharon Nademanee

Solomon Henry Stonebloom

Colin Scott Maxwell

Melissa Netwal

Elizabeth Lauren Meier

Stephen Blaskowski

Cameron Coates

Erik Jedediah Dean

Brian Chaikind

Kunal Mehta

Hendrik M Van Rossum

Paul Perkovich

The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Iterative genome editing in microbes

Methods, compositions, and kits for high throughput DNA assembly reactions in vitro. Modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa.

Applications of CRISPRi in high throughput metabolic engineering

The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.

Multiplexed deterministic assembly of DNA libraries

The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Pooled genome editing in microbes

Growing community of inventors

Berkeley, CA, United States of America

Aaron Miller