Medtronic Minimed, Inc.

Other

Aaron M Heiss

David J Vachon

Glenn Noronha

Joseph C Walsh

William P Van Antwerp

Bill C Ponder

Jonathan Reilly

John Joseph Mastrototaro

Thomas Arnold Peyser

Joe H Satcher, Jr

Stephen M Lane

Brooks B Cochran

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition/binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a 'switch' element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally 'off' in the absence of bound polyhydroxylate analyte and is generally &#x201c;on&#x201d; in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch &#x201c;on&#x201d; and &#x201c;off&#x201d;. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Analyte sensing via acridine-based boronate biosensors

Improved polymer matrices which incorporate fluorescent biosensor molecules as well as methods of making and using these polymer matrices are described. Such matrices can be used in fluorescent biosensors and biosensor systems, including those which are used in the detection of polyhydroxylated analytes such as glucose. The properties of the polymer matrices of the invention renders biosensors utilizing such matrices particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Polymers functionalized with fluorescent boronate motifs and methods for making them

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers

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Sherman Oaks, CA, United States of America

Aaron M Heiss