Illumina Corporation

Illumina Cambridge Limited

Aaron Liu

Stanley S Hong

Danilo Condello

M Shane Bowen

Dietrich A Dehlinger

Peter Clarke Newman

Mark R Pratt

Simon Prince

Merek C Siu

Alex Nemiroski

Frederick G Erie

Shaoping Lu

Yinghua Sun

Marco A Krumbuegel

Anthony Lam

Bojan Obradovic

Geraint Wyn Evans

Gary Mark Skinner

A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.

Illumination for fluorescence imaging using objective lens

The disclosure provides for structured illumination microscopy (SIM) imaging systems. In one set of implementations, a SIM imaging system may be implemented as a multi-arm SIM imaging system, whereby each arm of the system includes a light emitter and a beam splitter (e.g., a transmissive diffraction grating) having a specific, fixed orientation with respect to the system's optical axis. In a second set of implementations, a SIM imaging system may be implemented as a multiple beam splitter slide SIM imaging system, where one linear motion stage is mounted with multiple beam splitters having a corresponding, fixed orientation with respect to the system's optical axis. In a third set of implementations, a SIM imaging system may be implemented as a pattern angle spatial selection SIM imaging system, whereby a fixed two-dimensional diffraction grating is used in combination with a spatial filter wheel to project one-dimensional fringe patterns on a sample.

Dual optical grating slide structured illumination imaging

Pattern angle spatial selection structured illumination imaging

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Riverside, CA, United States of America

Aaron Liu